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Lignin degradation of banana stem waste by fungi Phanerochaete chrysosporium : effect of temperature and agitation/ Rubaiyi Mat Zaid

By:

Rubaiyi Mat Zaid

Material type: Text

TextPublication details: Kuantan, Pahang : UMP, 2009Description: 33p. : ill. (some col.) ; 30 cm. + 1 computer discISBN:

THE0004466(Local)

Other title:

Lignin degradation of banana stem waste by fungi Phanerochaete chrysosporium : effect of temperature and agitation [computer file]

Subject(s):

Dissertation note: Project paper (Bachelor of of Chemical engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009 Abstract: Banana stem contain 20-30 percent lignin and 60 percent cellulose. Phanerochaete chrysosporium is used to degrade the lignin to get the cellulose and hemicelluloses. Then, the cellulose and hemicelluloses will be converted to glucose by another strain. In this research, the variables studied are temperature (34oC, 40oC) and agitation rate (120 rpm, 200 rpm). The optimum temperature and agitation rate for P. chrysosporium as obtained from the literature are temperature 37oC and agitation rate 160 rpm. These set-point value were tested would gave the value of 52.9% of lignin degradation within 2 days of aerobic fermentation. A 22 factorial experiment including the variables temperature and agitation rate was designed around this optimum point. Yates’ method is used to calculate the effect of temperature, effect of agitation and the interactive effect of temperature and agitation. The result showed that the highest lignin degradation was obtained at T=37oC, 160 rpm and when the temperature and agitation were adjusted the percentage of lignin degraded is 5% only. Lignin degradation by P. chrysosporium did not favor high temperature and high agitation rate where at high temperature and agitation rate lignin degradation was very low. As conclusion, this study showed that P. chrysosporium is a very effective lignin degrader and sensitive towards changed in temperature and agitation rate.-Author-

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Holdings ( 2 )
Title notes ( 3 )

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Final Year Report	UMPLIB GAMBANG	TP248.27.F86 R83 2009 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000043083
Final Year Report	UMPLIB GAMBANG	CD 4073 \| TP248.27.F86 R83 2009 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000043084

Project paper (Bachelor of of Chemical engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009

Bibliography : p. 28-29

Banana stem contain 20-30 percent lignin and 60 percent cellulose. Phanerochaete chrysosporium is used to degrade the lignin to get the cellulose and hemicelluloses. Then, the cellulose and hemicelluloses will be converted to glucose by another strain. In this research, the variables studied are temperature (34oC, 40oC) and agitation rate (120 rpm, 200 rpm). The optimum temperature and agitation rate for P. chrysosporium as obtained from the literature are temperature 37oC and agitation rate 160 rpm. These set-point value were tested would gave the value of 52.9% of lignin degradation within 2 days of aerobic fermentation. A 22 factorial experiment including the variables temperature and agitation rate was designed around this optimum point. Yates’ method is used to calculate the effect of temperature, effect of agitation and the interactive effect of temperature and agitation. The result showed that the highest lignin degradation was obtained at T=37oC, 160 rpm and when the temperature and agitation were adjusted the percentage of lignin degraded is 5% only. Lignin degradation by P. chrysosporium did not favor high temperature and high agitation rate where at high temperature and agitation rate lignin degradation was very low. As conclusion, this study showed that P. chrysosporium is a very effective lignin degrader and sensitive towards changed in temperature and agitation rate.-Author-