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Separation of fructosyltransferase using ultrafiltration membrane : effect of pH and ionic strength on flux and rejection / Mohd Khairul Afizan Harun

By:

Mohd Khairul Afizan Harun

Material type: Text

TextPublication details: Kuantan, Pahang : UMP, 2009Description: ix, 98 p. : ill. (some col.) ; 30 cm. + 1 computer discISBN:

THE0004428(Local)

Other title:

Separation of fructosyltransferase using ultrafiltration membrane : effect of pH and ionic strength on flux and rejection [electronic resource]

Subject(s):

Dissertation note: Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2009 Abstract: There are various methods to separate between macro molecule and non dissolved particle in chemical process. One of the methods is separation process using ultrafiltarion membrane. In filtration process, the macromolecules such as enzyme will be retained on the membrane surface. This experiment is study about fouling characteristic occur in an industry. The fouled membrane surface problem gives the high cost operation and reduces the quality of production. Therefore, the main objectives for this experiment are to determine the effect of pH and ionic strength on membrane flux and rejection during fructosyltransferase (FTase) separation. The 50 kDa molecular weight cut off (MWCO) of ultrafiltration membrane was used during this experiment. Cross flow filtration was used to run this experiment in the lab scale. Total organic carbon (TOC) was used to analysis the concentration of sample. Potassium dihydrogen phosphate (KH2PO4) and dipotassium hydrogen phosphate buffer solution range pH 5 to pH 8 was applied to find the effect of pH and various molarities of NaCl (0.5M to 2.0M) was used to find the effect of ionic strength in ultrafiltration membrane. The experimental result shows that the optimum pH and ionic strength was 8.0 and 0.5M, respectively, in order to separate the FTase solution using ultrafiltration membrane.

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Holdings ( 2 )
Title notes ( 3 )

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Final Year Report	UMPLIB GAMBANG	TP248.25.M46 K43 2009 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000042879
Final Year Report	UMPLIB GAMBANG	CD 3967 \| TP248.25.M46 K43 2009 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000042880

Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2009

Bibliography : p. [58]-60

There are various methods to separate between macro molecule and non dissolved particle in chemical process. One of the methods is separation process using ultrafiltarion membrane. In filtration process, the macromolecules such as enzyme will be retained on the membrane surface. This experiment is study about fouling characteristic occur in an industry. The fouled membrane surface problem gives the high cost operation and reduces the quality of production. Therefore, the main objectives for this experiment are to determine the effect of pH and ionic strength on membrane flux and rejection during fructosyltransferase (FTase) separation. The 50 kDa molecular weight cut off (MWCO) of ultrafiltration membrane was used during this experiment. Cross flow filtration was used to run this experiment in the lab scale. Total organic carbon (TOC) was used to analysis the concentration of sample. Potassium dihydrogen phosphate (KH2PO4) and dipotassium hydrogen phosphate buffer solution range pH 5 to pH 8 was applied to find the effect of pH and various molarities of NaCl (0.5M to 2.0M) was used to find the effect of ionic strength in ultrafiltration membrane. The experimental result shows that the optimum pH and ionic strength was 8.0 and 0.5M, respectively, in order to separate the FTase solution using ultrafiltration membrane.