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Comparison between mehod of one-factor-at-a-time (OFAT) & design of experiment (DOE) in screening of immunoglobulin production stimulatesing factors / Muhd Abd Mursyid W. Mohd. Saberi

By:

Muhd Abd Mursyid W. Mohd. Saberi

Material type: Text

TextPublication details: Kuantan, Pahang : UMP, 2010Description: xii, 39 p. : ill. ; 30 cm. (some col.) + 1 CD-ROMISBN:

THE0004390(Local)

Subject(s):

Dissertation note: Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010 Abstract: The medium of mouse-mouse hybridoma, 192 producing IgG against 17-OHP was supplemented with seven different inducing agents at different range namely lysozyme, lipopolysaccharides (LPS), potassium phosphate, aldolase, essential amino acid, non-essential amino acid and sodium butyrate. This study aims to compare the statistical design of experiments (DOE) and one-factor-at-a-time (OFAT) method in screening immunoglobulin production stimulating factors. The culture medium supplemented with the inducer agents were screened using OFAT method and Plakett- Burman design method to determine inducer agent that gave positive effect. The effect of inducer concentration towards the antibody production was studied using enzyme-linked immunosorbent assay (ELISA) technique. The optimal concentrations for all the inducing agent were failed to be found in OFAT method. The standard culture medium without addition of inducer yield 0.234μg/ml of MAb. The culture supplemented with the inducer agent that yields MAb close to the standard was non-essential amino acid. Plakett-Burman design gave 12 runs with only the high level (+) and the low level (-) of the inducer were used. Potassium phosphate, LPS and sodium butyrate was found to be the most influential variable using Plakett-Burman design. Plakett-Burman design however indicates that all the point was insignificant and the interaction between inducer was not described. Plakett-Burman under DOE method gave the best time-wise compared to OFAT method in screening monoclonal antibody stimulating factors.

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Title notes ( 3 )

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Final Year Report	UMPLIB GAMBANG	TP248.25.C44 M87 2010 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000061018
Final Year Report	UMPLIB GAMBANG	CD 5672 \| TP248.25.C44 M87 2010 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000061019

Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010

Bibliography : p.36-38

The medium of mouse-mouse hybridoma, 192 producing IgG against 17-OHP was supplemented with seven different inducing agents at different range namely lysozyme, lipopolysaccharides (LPS), potassium phosphate, aldolase, essential amino acid, non-essential amino acid and sodium butyrate. This study aims to compare the statistical design of experiments (DOE) and one-factor-at-a-time (OFAT) method in screening immunoglobulin production stimulating factors. The culture medium supplemented with the inducer agents were screened using OFAT method and Plakett- Burman design method to determine inducer agent that gave positive effect. The effect of inducer concentration towards the antibody production was studied using enzyme-linked immunosorbent assay (ELISA) technique. The optimal concentrations for all the inducing agent were failed to be found in OFAT method. The standard culture medium without addition of inducer yield 0.234μg/ml of MAb. The culture supplemented with the inducer agent that yields MAb close to the standard was non-essential amino acid. Plakett-Burman design gave 12 runs with only the high level (+) and the low level (-) of the inducer were used. Potassium phosphate, LPS and sodium butyrate was found to be the most influential variable using Plakett-Burman design. Plakett-Burman design however indicates that all the point was insignificant and the interaction between inducer was not described. Plakett-Burman under DOE method gave the best time-wise compared to OFAT method in screening monoclonal antibody stimulating factors.