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Effect of substrate concentration and ph on succinic acid production / Nik Nor Aziati Abd Aziz

By:

Nik Nor Aziati Abd Aziz

Material type: Text

TextPublication details: Kuantan, Pahang : UMP, 2010Description: xv, 95 p. : ill. (some col.) ; 30 cm. + 1 CD-ROMISBN:

THE0004133(Local)

Subject(s):

Dissertation note: Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010 Summary: The purpose of this research is to study the effect of substrate concentration and pH on succinic acid production. Succinic acid has been produced from the fermentation process by using Escherichia coli b-strain. The experiments were conducted in Modified Schott Bottle in incubator shaker to maintain anaerobic condition, temperature and agitation speed. Fermentation were carried out at the optimum temperature of E.coli growth which is 37 OC for 72 hours. The results of HPLC analysis showed that increase in fermentation time will increase succinic acid production. The maximum production of succinic acid is at pH 6.0. Initial substrate concentration (5 to 15 g/L) will increase Succinic acid production, but after 15 g/L substrate concentration, the succinic acid production will decrease. From this study, it was observed that the substrate concentration and pH are important factor that affects both growth and growth-associated productions of molecules to increased succinic acid production.

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Holdings ( 2 )
Title notes ( 3 )

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Final Year Report	UMPLIB GAMBANG	TP156.F4 N67 2010 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000063206
Final Year Report	UMPLIB GAMBANG	CD 5876 \| TP156.F4 N67 2010 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000063207

Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010

Bibliography: p. 60-62

The purpose of this research is to study the effect of substrate concentration and pH on succinic acid production. Succinic acid has been produced from the fermentation process by using Escherichia coli b-strain. The experiments were conducted in Modified Schott Bottle in incubator shaker to maintain anaerobic condition, temperature and agitation speed. Fermentation were carried out at the optimum temperature of E.coli growth which is 37 OC for 72 hours. The results of HPLC analysis showed that increase in fermentation time will increase succinic acid production. The maximum production of succinic acid is at pH 6.0. Initial substrate concentration (5 to 15 g/L) will increase Succinic acid production, but after 15 g/L substrate concentration, the succinic acid production will decrease. From this study, it was observed that the substrate concentration and pH are important factor that affects both growth and growth-associated productions of molecules to increased succinic acid production.