Isolation, characterization and identification of microorganism from soil contaminated with pesticide / Adawiyah Ismail

Project paper (Bachelor of Chemical Engineering) --- Universiti Malaysia Pahang - 2008

This study is investigated the type of microorganisms that have a history of been treated of pesticide. When the microorganisms can survive in pollutant environment, it has potential to degrade substance that harmful to our environment. This is because environmental pollution, caused by the leaking of chemical fertilizers and pesticides to surface and groundwater, caused serious environmental and social problems throughout the world. The soil samples had been taken from dragon fruit plantation that has history in using of pesticide. Isolation of these microorganisms until get single colony had been done by using serial dilution method and streaking method. For the identification of these microorganisms, several biochemical identification methods such as Gram stain, Starch Hydrolysis and Triple sugar–Iron Agar Test h ad been used. As a result, four single colonies that represent Cocci in their morphology had been isolated. Sample 1 and 2 show its characteristic as gram negative in gram staining, meanwhile for Sample 3 and 4, its represent as gram positive. There were starch hydrolysis for Sample 1 and 4, but no starch hydrolysis for Sample 2 and 3. At the end of this study, Sample 1 and Sample 2 can be classified as Neisseria or Veillonella species. Meanwhile, Sample 3 and Sample 4 can be classified as Micrococcus sp or Staphylococcus sp. For further study, all of these samples can be introduce into bioremediation technique to study its potential in degrading pesticide. -Author