Effect of ionic strength on whey protein separation in high performance tangential flow filtration / Muhammad Fakhrullah Mohd Nor

By: Material type: TextTextPublication details: Kuantan : UMP, 2010Description: xiv, 46 p. : ill. (some col.) ; 30 cm. + 1 CD-ROMISBN:
  • THE0004524(Local)
Subject(s): Dissertation note: Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010 Abstract: High performance tangential flow filtration (HPTFF) is an emerging technology developed from the conventional cross flow filtration (CFF). HPTFF could separate protein components that differ less than three fold in size compared to the CFF, which only applicable to separate the protein components that differ more than tenfold in size. In the current study, HPTFF was operated using PES membrane with MWCO of 30kDa to separate whey protein components into two fractions which is enriched in α-lactalbumin (α-lac) on the permeate side and β-lactoglobulin (β-lg) in the retentate side. In order to get higher permeate value of α-lac, ionic strength of the solution from 0M to 0.5M in HPTFF was studied in this study. The best separation factor was observed at intermediate salt concentration of 0.125M concentration.
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Item type Current library Call number Copy number Status Date due Barcode
Final Year Report Final Year Report UMPLIB GAMBANG TP248.65.P76 F35 2010 rs Bc. (Browse shelf(Opens below)) 1 Not for loan 0000060206
Final Year Report Final Year Report UMPLIB GAMBANG CD 5497 | TP248.65.P76 F35 2010 rs Bc. (Browse shelf(Opens below)) 1 Not for loan 0000060207

Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010

High performance tangential flow filtration (HPTFF) is an emerging technology developed from the conventional cross flow filtration (CFF). HPTFF could separate protein components that differ less than three fold in size compared to the CFF, which only applicable to separate the protein components that differ more than tenfold in size. In the current study, HPTFF was operated using PES membrane with MWCO of 30kDa to separate whey protein components into two fractions which is enriched in α-lactalbumin (α-lac) on the permeate side and β-lactoglobulin (β-lg) in the retentate side. In order to get higher permeate value of α-lac, ionic strength of the solution from 0M to 0.5M in HPTFF was studied in this study. The best separation factor was observed at intermediate salt concentration of 0.125M concentration.

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