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Production of beta-carotene by Dunaliella Salina : effect of NaNO3 concentration and light intensity / Nur Isma Idayu Ismail

By:

Nur Isma Idayu Ismail

Material type: Text

TextPublication details: Kuantan, Pahang : UMP, 2012Description: xv, 36 p. : ill. ; 30 cm. + 1 CD-ROMISBN:

THE0002262(Local)

Subject(s):

Dissertation note: Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2012 Abstract: Beta-carotene is used as vitamin A precursor and its antioxidant property has been used in dietary supplements for the prevention of diseases such as cancer and coronary heart disease. The purpose of this study is to study the effect of NaNO3 concentration and light intensity to the growth and production of beta-carotene by Dunaliella salina. Four different NaNO3 concentrations (100%, 75%, 50%, and 25% v/w) and light intensities (6000 lx, 8000 lx, 11000 lx and 17000 lx) were used. Cell growth was determined by cell counting method and analysis of total beta-carotene concentration in the samples was done using High Performance Liquid Chromatography (HPLC). From the result obtained, the NaNO3 concentration and light intensity will affect the growth of Dunaliella salina, where the growth was reduced due to NaNO3 starvation and it was also induced with the high light intensity until nitrate became the limiting source for the cells. Besides that, NaNO3 concentration and light intensity will also affect the beta-carotene production of Dunaliella salina, where the production was increased as the NaNO3 concentration was decreased and the light intensity was increased until the concentration of NaNO3 has become the limiting factor of the experiment. In order to improve the research, further study to identify the optimal NaNO3 concentration and light intensity required for maximizing the production of beta-carotene by Dunaliella salina shall be done.

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Holdings ( 2 )
Title notes ( 3 )

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Final Year Report	UMPLIB GAMBANG	QP671.C3 I86 2012 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000074875
Final Year Report	UMPLIB GAMBANG	CD 7137 \| QP671.C3 I86 2012 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000074876

Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2012

Bibliography : p. 29-33

Beta-carotene is used as vitamin A precursor and its antioxidant property has been used in dietary supplements for the prevention of diseases such as cancer and coronary heart disease. The purpose of this study is to study the effect of NaNO3 concentration and light intensity to the growth and production of beta-carotene by Dunaliella salina. Four different NaNO3 concentrations (100%, 75%, 50%, and 25% v/w) and light intensities (6000 lx, 8000 lx, 11000 lx and 17000 lx) were used. Cell growth was determined by cell counting method and analysis of total beta-carotene concentration in the samples was done using High Performance Liquid Chromatography (HPLC). From the result obtained, the NaNO3 concentration and light intensity will affect the growth of Dunaliella salina, where the growth was reduced due to NaNO3 starvation and it was also induced with the high light intensity until nitrate became the limiting source for the cells. Besides that, NaNO3 concentration and light intensity will also affect the beta-carotene production of Dunaliella salina, where the production was increased as the NaNO3 concentration was decreased and the light intensity was increased until the concentration of NaNO3 has become the limiting factor of the experiment. In order to improve the research, further study to identify the optimal NaNO3 concentration and light intensity required for maximizing the production of beta-carotene by Dunaliella salina shall be done.