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Total phenolic content and antioxidant activity of some Malaysian herbs / Norhayantie A. Rahman

By:

Norhayantie A. Rahman

Material type: Text

TextPublication details: Kuantan, Pahang : UMP, 2013Description: xvi, 69 p. : ill. ; 30 cm. + 1 CD-ROMISBN:

THE0002153(Local)

Subject(s):

Dissertation note: Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013 Abstract: Herbs have drawn much attention of people worldwide, not only because of their economic value as food products, but also for their antioxidant compounds. Thus, this study was conducted to investigate the total phenolic content and antioxidant activities on three Malaysian herbs by using Soxhlet extraction method. The herbs were Eugenia polyantha (Serai kayu), Euodia redlevi (Tenggek burung) and Limnocharis flava (Sudu itik). The total phenolic content and antioxidant compounds were extracted using distilled water at three different extraction time (4, 8 and 12 hour). The extracts were then evaluated using Folin-Ciocalteu reagent for their total phenolic content and 2, 2-Diphenyl-1-picrylhydrazyl hydrate (DPPH) assay for their antioxidant compounds. Further analyzed was done using High Performance Liquid Chromatography (HPLC) to further verifying the ascorbic acid existence in the extracts. It was found L. flava showed the highest content of total phenolic compounds (15.01 mg gallic acid equivalent (GAE) per 1 g of sample) with the antioxidant capacity standing at 20.42 mg ascorbic acid equivalent (AAE) per 1 g of sample after 8 hour extraction. While, E. polyantha showed the highest value of antioxidant capacity (21.19 mg AAE/g) with total phenolic content of 9.99 mg GAE/g. In HPLC analysis, it was found that E. polyantha and L. flava after 8 hour extraction contained 0.7625 and 0.9745 mg AAE/g of ascorbic acid respectively.

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Title notes ( 3 )

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Final Year Report	UMPLIB GAMBANG	QD341.P5 H39 2013 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000074749
Final Year Report	UMPLIB GAMBANG	CD 7074 \| QD341.P5 H39 2013 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000074750

Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013

Bibliography : p. 54-59

Herbs have drawn much attention of people worldwide, not only because of their economic value as food products, but also for their antioxidant compounds. Thus, this study was conducted to investigate the total phenolic content and antioxidant activities on three Malaysian herbs by using Soxhlet extraction method. The herbs were Eugenia polyantha (Serai kayu), Euodia redlevi (Tenggek burung) and Limnocharis flava (Sudu itik). The total phenolic content and antioxidant compounds were extracted using distilled water at three different extraction time (4, 8 and 12 hour). The extracts were then evaluated using Folin-Ciocalteu reagent for their total phenolic content and 2, 2-Diphenyl-1-picrylhydrazyl hydrate (DPPH) assay for their antioxidant compounds. Further analyzed was done using High Performance Liquid Chromatography (HPLC) to further verifying the ascorbic acid existence in the extracts. It was found L. flava showed the highest content of total phenolic compounds (15.01 mg gallic acid equivalent (GAE) per 1 g of sample) with the antioxidant capacity standing at 20.42 mg ascorbic acid equivalent (AAE) per 1 g of sample after 8 hour extraction. While, E. polyantha showed the highest value of antioxidant capacity (21.19 mg AAE/g) with total phenolic content of 9.99 mg GAE/g. In HPLC analysis, it was found that E. polyantha and L. flava after 8 hour extraction contained 0.7625 and 0.9745 mg AAE/g of ascorbic acid respectively.