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Optimization of pre-treatment process of glycerol residue for succinic acid production / Nik Nor Aziati Abd Aziz

By:

Nik Nor Aziati Abd Aziz

Material type: Text

TextPublication details: Kuantan, Pahang : UMP, 2013Description: xx, 132 p. : ill. (some col.) ; 30 cm. + 1 CD-ROMISBN:

THE0004903(Local)

Subject(s):

Glycerin

Online resources:

Library access only

Dissertation note: Thesis (Master of Engineering in Bio-Process) -- Universiti Malaysia Pahang - 2013 Abstract: The purpose of this study is to recover glycerol from the oleo-chemical industry waste through acid pre-treatment process. Pre-treatment intervals parameters (pH, temperature and mass substrate) was obtained from OFAT, while the optimum conditions for the recovery of glycerol obtained by using response surface methodology (RSM). Glycerol concentrations before and after pre-treatment and succinic acid derived from the fermentation process were analyzed using HPLC. FTIR spectrometry was used to identify functional groups of glycerol. The optimum conditions of pH, temperature and substrate mass for glycerol pre-treatment process is at 2.0, 35 ° C and 110.3600 grams respectively. In optimal conditions, the concentration of glycerol after pre-treatment process is 159.3120 g / L. Glycerol is then used in the fermentation by Escherichia coli K-12 types to produce succinic acid of 0.6698 g / L, with initial concentration of glycerol, rotational speed and temperature at 19.6700 g / L, 200 rpm and 37 ° C, respectively. Succinic acid production from glycerol derived from the recovery process is 8.67% lower than the production of succinic acid from commercial glycerol. Therefore, this study was able to demonstrate that the glycerol from the oleo-chemical industry waste can be used in the production of succinic acid.

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Holdings ( 2 )
Title notes ( 3 )

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Thesis	UMPLIB GAMBANG	TP973 .A95 2013 Thesis (Browse shelf(Opens below))	1	Not for loan		0000080425
Thesis	UMPLIB GAMBANG	CD 7312 \| TP973 .A95 2013 Thesis (Browse shelf(Opens below))	1	Not for loan		0000076102

Thesis (Master of Engineering in Bio-Process) -- Universiti Malaysia Pahang - 2013

Bibliography : p. 106-113

The purpose of this study is to recover glycerol from the oleo-chemical industry waste through acid pre-treatment process. Pre-treatment intervals parameters (pH, temperature and mass substrate) was obtained from OFAT, while the optimum conditions for the recovery of glycerol obtained by using response surface methodology (RSM). Glycerol concentrations before and after pre-treatment and succinic acid derived from the fermentation process were analyzed using HPLC. FTIR spectrometry was used to identify functional groups of glycerol. The optimum conditions of pH, temperature and substrate mass for glycerol pre-treatment process is at 2.0, 35 ° C and 110.3600 grams respectively. In optimal conditions, the concentration of glycerol after pre-treatment process is 159.3120 g / L. Glycerol is then used in the fermentation by Escherichia coli K-12 types to produce succinic acid of 0.6698 g / L, with initial concentration of glycerol, rotational speed and temperature at 19.6700 g / L, 200 rpm and 37 ° C, respectively. Succinic acid production from glycerol derived from the recovery process is 8.67% lower than the production of succinic acid from commercial glycerol. Therefore, this study was able to demonstrate that the glycerol from the oleo-chemical industry waste can be used in the production of succinic acid.