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Yeast protocols / edited by Wei Xiao

Contributor(s): Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; 313Publication details: Totowa, N.J. : Humana Press, c2006Edition: 2nd edDescription: xi, 392 p. : ill. ; 23 cmISBN:
  • 1588294374 (alk. paper)
  • 9781588294371
Subject(s): Online resources:
Contents:
Basic investigations in saccharomyces cerevisiae / Brendan P.G. Curran and Virginia Bugeja -- Isolation of nucleic acids / Michelle Hanna and Wei Xiao -- Purification of yeast peroxisomes / Ben Distal and Astrid Kragt -- Isolation of yeast plasma membranes / Barry Panaretou and Peter Piper -- Isolation of yeast mitochondria / Chris Meisinger, Nikolaus Pfanner, and Kaye N. Truscott -- Extraction of yeast lipids / Roger Schneiter and Günther Daum -- Two-dimensional gel electrophoresis of total yeast proteins / Hélian Boucherie and Christelle Monribot-Espagne -- Pulsed field gel electrophoresis of budding yeast chromosomes / Laura Maringele and David Lydall -- Analysis of yeast lipids / Roger Schneiter and Günther Daum -- Yeast fluorescence microscopy / Jirí Hasek --
Intracellular expression of recombinant antibody fluorescent protein fusions for localization of target antigens in schizosaccharomyces pombe / Michelle A. Alting-Mees ... [et al.] -- Yeast transformed by the LiAc/SS carrier DNA/PEG method / R. Daniel Gietz and Robin A. Woods -- Mutagenesis / Leslie Barbour, Michelle Hanna, and Wei Xiao -- Gene disruption in the budding yeast saccharomyces cerevisiae / Johannes H. Hegemann, Ulrich Güldener, and Gabriele J. Köhler -- Inducible degron and its application to creating conditional mutants / R. Jürgen Dohmen -- Synthetic lethal screen / Leslie Barbour and Wei Xiao -- Synthetic genetic array analysis in saccharomyces cerevisiae / Amy Hin Yan Tong and Charles Boone -- Two-dimensional agarose gel analysis of DNA replication intermediates / Alain T. Dandjinou ... [et al.] -- Chromatin assembly in a crude fraction from yeast cells / Karen M. Robinson and Michael C. Schultz -- Chromatin immunoprecipitation to study protein/DNA interactions in budding yeast / Elena Ezhkova and William P. Tansey --
Isolation of yeast nuclei and micrococcal nuclease mapping of nucleosome positioning / Zhengjian Zhang and Joseph C. Reese -- Study of transcriptional regulation using a reporter gene assay / Yu Fu and Wei Xiao -- Assessing telomeric phenotypes / Catherine LeBel ... [et al.] -- Controlled expression of recombinant genes and preparation of cell-free extracts in yeast / Zhigang Wang -- Production of heterologous proteins in yeast with the aid of the Hsp150-Carrier / Marja Makarow ... [et al.] -- Yeast two-hybrid system screening / R. Daniel Gietz -- Import of precursor proteins into isolated yeast mitochondria / Nils Wiedemann, Nikolaus Pfanner, and Peter Rehling
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Holdings
Item type Current library Call number Copy number Status Date due Barcode
Open Shelf Open Shelf UMPLIB GAMBANG TP248.27.Y43 Y43 2006 (Browse shelf(Opens below)) 1 Available 0000046760

Includes bibliographical references and index

Basic investigations in saccharomyces cerevisiae / Brendan P.G. Curran and Virginia Bugeja -- Isolation of nucleic acids / Michelle Hanna and Wei Xiao -- Purification of yeast peroxisomes / Ben Distal and Astrid Kragt -- Isolation of yeast plasma membranes / Barry Panaretou and Peter Piper -- Isolation of yeast mitochondria / Chris Meisinger, Nikolaus Pfanner, and Kaye N. Truscott -- Extraction of yeast lipids / Roger Schneiter and Günther Daum -- Two-dimensional gel electrophoresis of total yeast proteins / Hélian Boucherie and Christelle Monribot-Espagne -- Pulsed field gel electrophoresis of budding yeast chromosomes / Laura Maringele and David Lydall -- Analysis of yeast lipids / Roger Schneiter and Günther Daum -- Yeast fluorescence microscopy / Jirí Hasek --

Intracellular expression of recombinant antibody fluorescent protein fusions for localization of target antigens in schizosaccharomyces pombe / Michelle A. Alting-Mees ... [et al.] -- Yeast transformed by the LiAc/SS carrier DNA/PEG method / R. Daniel Gietz and Robin A. Woods -- Mutagenesis / Leslie Barbour, Michelle Hanna, and Wei Xiao -- Gene disruption in the budding yeast saccharomyces cerevisiae / Johannes H. Hegemann, Ulrich Güldener, and Gabriele J. Köhler -- Inducible degron and its application to creating conditional mutants / R. Jürgen Dohmen -- Synthetic lethal screen / Leslie Barbour and Wei Xiao -- Synthetic genetic array analysis in saccharomyces cerevisiae / Amy Hin Yan Tong and Charles Boone -- Two-dimensional agarose gel analysis of DNA replication intermediates / Alain T. Dandjinou ... [et al.] -- Chromatin assembly in a crude fraction from yeast cells / Karen M. Robinson and Michael C. Schultz -- Chromatin immunoprecipitation to study protein/DNA interactions in budding yeast / Elena Ezhkova and William P. Tansey --

Isolation of yeast nuclei and micrococcal nuclease mapping of nucleosome positioning / Zhengjian Zhang and Joseph C. Reese -- Study of transcriptional regulation using a reporter gene assay / Yu Fu and Wei Xiao -- Assessing telomeric phenotypes / Catherine LeBel ... [et al.] -- Controlled expression of recombinant genes and preparation of cell-free extracts in yeast / Zhigang Wang -- Production of heterologous proteins in yeast with the aid of the Hsp150-Carrier / Marja Makarow ... [et al.] -- Yeast two-hybrid system screening / R. Daniel Gietz -- Import of precursor proteins into isolated yeast mitochondria / Nils Wiedemann, Nikolaus Pfanner, and Peter Rehling

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