Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration / Norhalmy B. Mohd Nor
Material type:
TextPublication details: Kuantan, Pahang : KUKTEM , 2006Description: xiii, 50 p. : ill. ; 30 cm. + 1 computer discISBN: - THE0004132(Local)
| Item type | Current library | Call number | Copy number | Status | Date due | Barcode | |
|---|---|---|---|---|---|---|---|
Final Year Report
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UMPLIB GAMBANG | TP156.F4 N67 2006 rs Thesis (Browse shelf(Opens below)) | 1 | Not for loan | 0000021546 | ||
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Final Year Report
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UMPLIB GAMBANG | CD 2039 | TP156.F4 N67 2006 rs Thesis (Browse shelf(Opens below)) | 1 | Not for loan | 0000021547 |
Project paper (Bachelor of Chemical Engineering) -- Kolej Universiti Kejuruteraan dan Teknologi Malaysia - 2006
Bibliography: p. 44-46
ABST ACT The use of waste as raw material is important for government economy and natural balance. The purpose of this work is to study the production of xylanase and CMCase enzyme by Aspergillus niger in solid state fermentation (SSF) using agricultural residues sugar cane bagasse as substrates. The study is focus on the effect of substrate concentration which is 1 g/L, 3g/L and 5 g/L. All the enzymes were incubating in temperature 37 oC and pH 5.0 for 30 minutes. The reaction was quenched by cooling on ice and the amount of reducing sugar was measured by dinitrosalicyclic (DNS) acid procedure with slight modification (Miller, 1965). The mixture was then incubated at 100 oC for 5 minutes. Subsequently, the reaction was stopped by cooling on ice. Distilled water was added into the final volume of 16 ml and the color intensity was measured at wavelength of 575 nm. The concentration of SCB affected the xylanase and CMCase activity. Xylanase and carboxymethylcellulase activity was higher at lower concentration hen using 3 g of SCB