Effect of substrate concentration on profile growth of candida tropicalis ifo 0618 / Siti Nur Hatika Abu Bakar

By: Material type: TextTextPublication details: Kuantan, Pahang : UMP, 2014Description: xv, 78 p. : ill. ; 30 cm. + 1 CD-ROMISBN:
  • THE0002247(Local)
Subject(s): Dissertation note: Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2014 Abstract: This study presents effect of substrate concentration on the profile growth of Candida tropicalis IFO 0618. Different substrate concentration; D-Glucose were introduced to Candida tropicalis IFO 0618 in the Solid State Fermentation (SSF) process. The effect of substrate concentration on the profile growth of Candida tropicalis IFO 0618 was analyzed using UV-Vis Spectrophotometer and High Performance Liquid Chromatography (HPLC). The kinetic parameter (μmax) was also calculated to describe the microbial growth. From the laboratory data, the specific growth rate (μ), maximum specific growth rate (μmax) and half saturation coefficients (KS) were determined using the Monod equation. The maximum observed growth rate (μmax) for Candida tropicalis IFO 0618 was at the substrate concentration of 8.5 (g/g) with μmax value of 1.9991 hr-1, specific growth rate (μ) of 0.0821 hr-1 and half saturation coefficients (KS) of 198.4663 g/l. Result from the High Performance Liquid Chromatography also shows that there was sorbitol produced at 8.5 (g/g) substrate concentrations
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Final Year Report Final Year Report UMPLIB GAMBANG QP605 .H38 2014 r Bc. (Browse shelf(Opens below)) 1 Not for loan 0000091699
Final Year Report Final Year Report UMPLIB GAMBANG CD 8616 | QP605 .H38 2014 r Bc. (Browse shelf(Opens below)) 1 Not for loan 0000091700

Faculty of Chemical & Natural Resources Engineering

Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2014

Bibliography : p. 70-75

This study presents effect of substrate concentration on the profile growth of Candida tropicalis IFO 0618. Different substrate concentration; D-Glucose were introduced to Candida tropicalis IFO 0618 in the Solid State Fermentation (SSF) process. The effect of substrate concentration on the profile growth of Candida tropicalis IFO 0618 was analyzed using UV-Vis Spectrophotometer and High Performance Liquid Chromatography (HPLC). The kinetic parameter (μmax) was also calculated to describe the microbial growth. From the laboratory data, the specific growth rate (μ), maximum specific growth rate (μmax) and half saturation coefficients (KS) were determined using the Monod equation. The maximum observed growth rate (μmax) for Candida tropicalis IFO 0618 was at the substrate concentration of 8.5 (g/g) with μmax value of 1.9991 hr-1, specific growth rate (μ) of 0.0821 hr-1 and half saturation coefficients (KS) of 198.4663 g/l. Result from the High Performance Liquid Chromatography also shows that there was sorbitol produced at 8.5 (g/g) substrate concentrations

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