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Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE) / Nur 'Izzaty Hazera Nazar

By:

Nur 'Izzaty Hazera Nazar [author.]

Material type: Text

text
text

Media type:

unmediated
computer

Carrier type:

volume
computer disc

ISBN:

THE0000923(Local)

Subject(s):

Dissertation note: Project Paper (Bachelors of Chemical Engineering) -- Universiti Malaysia Pahang – 2017 Abstract: Cyclodextrin glycosyltransferase (CGTase) is an enzyme that produces cyclodextrin (CD) from starch under catalytic reaction. It is an important industrial enzyme which mostly used in food and pharmaceutical industry. The purpose of this study is to determine the effect of operating conditions on characterization and stabilization of CGTase to improve the production of cyclodextrin (CD) in the future. Therefore, the effect of starch concentration and pH on the characterization of CGTase were studied. The effect of pH and temperature on stabilization of CGTase were investigated. The best starch concentration and pH on characterization of CGTase were 3% w.v and pH 6 respectively. The most stable conditions of CGTase were detected at pH 6-8 and temperature of 40-60˚C. By using these optimum conditions, the CD production may improve in the future which in turn may be beneficial to industrial uses.

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Holdings ( 2 )
Title notes ( 4 )

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Final Year Report	UMPLIB GAMBANG	FKKSA .I99 2017 r Bc. (Browse shelf(Opens below))	1	Not for loan		0000122144
Final Year Report	UMPLIB GAMBANG	CD 11152 \| FKKSA .I99 2017 r Bc. (Browse shelf(Opens below))	1	Not for loan		0000122145

Faculty of Chemical & Natural Resources Engineering

Project Paper (Bachelors of Chemical Engineering) -- Universiti Malaysia Pahang – 2017

Includes bibliographical references

Cyclodextrin glycosyltransferase (CGTase) is an enzyme that produces cyclodextrin (CD) from starch under catalytic reaction. It is an important industrial enzyme which mostly used in food and pharmaceutical industry. The purpose of this study is to determine the effect of operating conditions on characterization and stabilization of CGTase to improve the production of cyclodextrin (CD) in the future. Therefore, the effect of starch concentration and pH on the characterization of CGTase were studied. The effect of pH and temperature on stabilization of CGTase were investigated. The best starch concentration and pH on characterization of CGTase were 3% w.v and pH 6 respectively. The most stable conditions of CGTase were detected at pH 6-8 and temperature of 40-60˚C. By using these optimum conditions, the CD production may improve in the future which in turn may be beneficial to industrial uses.