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Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line / Wong Shin Yie

By:

Wong, Shin Yie [author.]

Material type: Text

TextPublisher: Kuantan, Pahang : UMP, 2017Description: xiii, 71 pages : illustrations (some color) ; 30 cm. + 1 CD-ROMContent type:

text

Media type:

unmediated
computer

Carrier type:

volume
computer disc

ISBN:

THE0001437(Local)

Subject(s):

Dissertation note: Project Paper (Bachelor Of Manufacturing Engineering Technology (Pharmaceutical) -- Universiti Malaysia Pahang – 2017 Abstract: Rapid increase in the demand for biopharmaceutical products has posed a challenge in the selection of high producing cell line that produce maximum amount of product. Various advances and efforts has been done on cell culture technology for Chinese Hamster Ovary (CHO) cell lines to achieve an improvement in the specific productivity of CHO cell lines. This study identifies the effects and relationship of various physiological characteristics of a panel of six CHO stable cell lines on the specific productivity (qp) of cell lines ranging from 2 to 50 pg/cell/day. We examined the correlation between physiological characteristics and specific productivity throughout the growth phase of batch culture. Results obtained were statistically analysed using Principal Component Analysis (PCA). Our research revealed that, overall, specific productivity is positively correlated to the total intracellular protein content of the cell lines. Meanwhile, specific productivity is negatively correlated to the cell density throughout the development of cell lines. However, this correlation was not observed at all-time points throughout the growth phase. Another significant finding from the research is that the correlation between physiological characteristics and specific productivity was the strongest during late exponential 5 of the growth phase as compared to early exponential.

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Holdings ( 2 )
Title notes ( 4 )

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Final Year Report	UMPLIB GAMBANG	FTeK .W66 2017 r Bc. (Browse shelf(Opens below))	1	Not for loan		0000122928
Final Year Report	UMPLIB GAMBANG	CD 11347 \| FTeK .W66 2017 r Bc. (Browse shelf(Opens below))	1	Not for loan		0000122929

Faculty of Engineering Technology

Project Paper (Bachelor Of Manufacturing Engineering Technology (Pharmaceutical) -- Universiti Malaysia Pahang – 2017

Includes bibliographical references

Rapid increase in the demand for biopharmaceutical products has posed a challenge in the selection of high producing cell line that produce maximum amount of product. Various advances and efforts has been done on cell culture technology for Chinese Hamster Ovary (CHO) cell lines to achieve an improvement in the specific productivity of CHO cell lines. This study identifies the effects and relationship of various physiological characteristics of a panel of six CHO stable cell lines on the specific productivity (qp) of cell lines ranging from 2 to 50 pg/cell/day. We examined the correlation between physiological characteristics and specific productivity throughout the growth phase of batch culture. Results obtained were statistically analysed using Principal Component Analysis (PCA). Our research revealed that, overall, specific productivity is positively correlated to the total intracellular protein content of the cell lines. Meanwhile, specific productivity is negatively correlated to the cell density throughout the development of cell lines. However, this correlation was not observed at all-time points throughout the growth phase. Another significant finding from the research is that the correlation between physiological characteristics and specific productivity was the strongest during late exponential 5 of the growth phase as compared to early exponential.