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Growth optimization of N-fixer, isolated from biofertilizer / Intan Noorleana Bt Rosdi

By:

Intan Noorleana Rosdi

Material type: Text

TextPublication details: Kuantan : UMP, 2009Description: 35 p. : ill. (some col.) ; 30 cm. + 1 computer discISBN:

THE0002279(Local)

Other title:

Growth optimization of n-fixer, isolated from [electronic resource]

Subject(s):

Bacterial growth

Dissertation note: Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009 Abstract: The objectives of this experiment were to obtain the optimum temperature, pH and the requirement of carbon sources needed for the growth of N-Fixer bacteria. This research can be divided into two parts which are the standard curve preparation followed by optimization of the experiments. Three standard curves needs to be constructed; growth, glucose and CFU curves. These three curves will be used in optimizing the growth condition of N-fixer. Set of experimental works were designed by DOE software using full factorial function. Based on the experiment results, it shows that the optimum pH and temperature for the growth of N-Fixer is at pH 6 and 30°C respectively. As for the requirement of carbon sources, 10 g/L was applicable to avoid the surplus glucose become toxic to microorganisms. As the conclusion, the concentration of glucose plays a major role in the fermentation process (18 hours) either to increase the number of N-fixer cell or to decrease it. Therefore, by maintaining it at 10g/L the number of cell can achieve the upper limit to where it can be produced which is about 1.6834E8 cell/mL .

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Holdings ( 2 )
Title notes ( 2 )

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Final Year Report	UMPLIB GAMBANG	CD 3924 \| QR84.5 .I58 2009 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000042794
Final Year Report	UMPLIB GAMBANG	QR84.5 .I58 2009 rs Bc. (Browse shelf(Opens below))	1	Not for loan		0000042793

Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009

The objectives of this experiment were to obtain the optimum temperature, pH and the requirement of carbon sources needed for the growth of N-Fixer bacteria. This research can be divided into two parts which are the standard curve preparation followed by optimization of the experiments. Three standard curves needs to be constructed; growth, glucose and CFU curves. These three curves will be used in optimizing the growth condition of N-fixer. Set of experimental works were designed by DOE software using full factorial function. Based on the experiment results, it shows that the optimum pH and temperature for the growth of N-Fixer is at pH 6 and 30°C respectively. As for the requirement of carbon sources, 10 g/L was applicable to avoid the surplus glucose become toxic to microorganisms. As the conclusion, the concentration of glucose plays a major role in the fermentation process (18 hours) either to increase the number of N-fixer cell or to decrease it. Therefore, by maintaining it at 10g/L the number of cell can achieve the upper limit to where it can be produced which is about 1.6834E8 cell/mL .