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Antimicrobial evaluation of herbosomal gel formulation from quercus infectoria extracts / Nurul Izzati Azahar

By:

Nurul Izzati Azahar [author.]

Material type: Text

text
text

Media type:

unmediated
computer

Carrier type:

volume
computer disc

ISBN:

THE0008956(Local)

Subject(s):

Dissertation note: Project Paper (Bachelor of Manufacturing Engineering Technology (Pharmaceutical)) -- Universiti Malaysia Pahang – 2019 Abstract: The antimicrobial medication contains synthetic ingredients which irritants the skin. Thus, instead of using synthetic antimicrobial ingredients, Q. infectoria are opted to treat microbes. Aside from that, poor absorption and bioavailability of herbal extracts and constituents may cause by poor solubility and improper size of the molecule. The formulation made for herbosomal gel used for the medicinal purpose by enhancing the bioavailability of herbal extract using phyto-pharmaceutical technique. This study is purposely made to formulate the herbosomal gel from Q. infectoria extract. The absorption of plant extracts in topical application on skin improved using extraction of herbosomal gel. The herbosomal gel has smooth without grittiness structure, having cooling sensation, acceptable viscosity and good appearance after 1 months of stability range. The Microbial Inhibition Concentration (MIC) is determined using serial dilution techniques. Both the gel formulation and the extract of Q. infectoria is diluted with sterile distilled water and 2.5% DMSO respectively. The concentration of serial dilution lays in range between 0.01 mg/ml to 5.00 mg/ml using half fold serial dilution. The nutrients broth is used as sterility control whereas bacterial broth as growth control. The antimicrobial testing is evaluated using gram-positive and gram-negative microorganisms by measuring the total surface area of bacterial inhibition on agar plate using agar well diffusion method. The nutrients plate is spread with 80 µl of bacterial broth. Then, the plate bore using 5mm tip to make a well. The well is later filled with gel formulation or extract of Q. infectoria. The plate is placed in incubator for 24 hours at 37º C. The inhibition zone for antimicrobial activity on nutrients agar plate shown that gram-positive bacteria have bigger inhibition area compare to gram-negative bacteria. The inhibition area for S. aureus and P. aeruginosa are 17.69 mm and 17.59 mm respectively. As for Q. infectoria extracts, the area of inhibition zones are 22.47 mm for S. aureus and 21.29 mm for P. aeruginosa.

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Holdings ( 2 )
Title notes ( 4 )

Holdings
Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode
Restricted Collection	UMPLIB PEKAN	Reference	FTKPM .I99 2019 r Bc. (Browse shelf(Opens below))	1	Not for loan		T000001237
Restricted Collection	UMPLIB PEKAN	Reference	CD12804 (Browse shelf(Opens below))	1	Not for loan		T000001238

Faculty of Manufacturing and Mechatronic Engineering Technology

Project Paper (Bachelor of Manufacturing Engineering Technology (Pharmaceutical)) -- Universiti Malaysia Pahang – 2019

Includes bibliographical references

The antimicrobial medication contains synthetic ingredients which irritants the skin. Thus, instead of using synthetic antimicrobial ingredients, Q. infectoria are opted to treat microbes. Aside from that, poor absorption and bioavailability of herbal extracts and constituents may cause by poor solubility and improper size of the molecule. The formulation made for herbosomal gel used for the medicinal purpose by enhancing the bioavailability of herbal extract using phyto-pharmaceutical technique. This study is purposely made to formulate the herbosomal gel from Q. infectoria extract. The absorption of plant extracts in topical application on skin improved using extraction of herbosomal gel. The herbosomal gel has smooth without grittiness structure, having cooling sensation, acceptable viscosity and good appearance after 1 months of stability range. The Microbial Inhibition Concentration (MIC) is determined using serial dilution techniques. Both the gel formulation and the extract of Q. infectoria is diluted with sterile distilled water and 2.5% DMSO respectively. The concentration of serial dilution lays in range between 0.01 mg/ml to 5.00 mg/ml using half fold serial dilution. The nutrients broth is used as sterility control whereas bacterial broth as growth control. The antimicrobial testing is evaluated using gram-positive and gram-negative microorganisms by measuring the total surface area of bacterial inhibition on agar plate using agar well diffusion method. The nutrients plate is spread with 80 µl of bacterial broth. Then, the plate bore using 5mm tip to make a well. The well is later filled with gel formulation or extract of Q. infectoria. The plate is placed in incubator for 24 hours at 37º C. The inhibition zone for antimicrobial activity on nutrients agar plate shown that gram-positive bacteria have bigger inhibition area compare to gram-negative bacteria. The inhibition area for S. aureus and P. aeruginosa are 17.69 mm and 17.59 mm respectively. As for Q. infectoria extracts, the area of inhibition zones are 22.47 mm for S. aureus and 21.29 mm for P. aeruginosa.