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Synthesis of cyclodextrin (CD) using immobilized enzyme on hollow fiber membrane/ Suhaily Suhaimi

By:

Suhaily Suhaimi [author.]

Material type: Text

text
text

Media type:

unmediated
computer

Carrier type:

volume

ISBN:

THE0008342(Local)

Subject(s):

Dissertation note: Thesis (Master of Engineering (Chemical)) -- Universiti Malaysia Pahang – 2019 Abstract: Cyclodextrin (CD) is produced by the enzymatic reaction of cyclodextrin glucanotransferase (CGTase) in the present of starch as a substrate. The CD has the ability to act as encapsulating agent and also additives in many applications. However, the used of CGTase is often limited due to the instability of the enzyme and easily denatured during the production of CD. Hence, enzyme immobilization has been widely explored to overcome these problems. Various immobilization methods and supports have been investigated over the years, yet less study was conducted in detail on the effect of reaction parameters for the production of CD by using immobilized CGTase. The objectives of this study were to determine and to optimize the effect of reaction parameters for the production of CD by using the immobilized CGTase on polyvinylidene difluoride (PVDF) hollow fiber membrane. The effect of starch type, starch concentration, temperature, pH, reaction time and agitation rate on the CD production were determined by using one factor at a time (OFAT). The results revealed that 3% (w/v) of soluble potato starch produced the highest amount of CD (5.22 mg/mL). The production of CD was also the highest at temperature of 40°C (5.21 mg/mL), pH 6 (4.62 mg/mL), 6 h of reaction time (4.37 mg/mL) and 150 rpm of agitation rate (4.38 mg/ml). From the result, immobilized CGTase managed to produce 2 to 3-fold higher of CD production than the free CGTase. The screening of the significant reaction parameters for the production of CD were conducted by using fractional factorial design (FFD). The results showed that the concentration of soluble potato starch concentration, pH and temperature were the most significant parameters on the production of CD. The reaction parameters were then optimized by using central composite design (CCD). Under the optimized conditions (3.2% w/v concentration of soluble potato starch, 45.2°C of reaction temperature and pH 5.6), the production of CD was 3.2-fold higher than the amount of CD before the optimization process. The immobilized enzyme was successfully retained up to 38% of the initial activity and can be reused for 10 cycles for the production of CD. Therefore, the immobilization of CGTase on hollow fiber membrane proved to be suitable to enhance the production of CD with high enzyme stability.

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Holdings ( 2 )
Title notes ( 4 )

Holdings
Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode
Thesis	UMPLIB GAMBANG	Reference	FKKSA .S84 2019 r Thesis (Browse shelf(Opens below))	1	Not for loan		T000000181
Thesis	UMPLIB GAMBANG	Reference	CD12256 (Browse shelf(Opens below))	1	Not for loan		T000000182

Faculty of Chemical and Natural Resources Engineering

Thesis (Master of Engineering (Chemical)) -- Universiti Malaysia Pahang – 2019

Includes bibliographical references

Cyclodextrin (CD) is produced by the enzymatic reaction of cyclodextrin glucanotransferase (CGTase) in the present of starch as a substrate. The CD has the ability to act as encapsulating agent and also additives in many applications. However, the used of CGTase is often limited due to the instability of the enzyme and easily denatured during the production of CD. Hence, enzyme immobilization has been widely explored to overcome these problems. Various immobilization methods and supports have been investigated over the years, yet less study was conducted in detail on the effect of reaction parameters for the production of CD by using immobilized CGTase. The objectives of this study were to determine and to optimize the effect of reaction parameters for the production of CD by using the immobilized CGTase on polyvinylidene difluoride (PVDF) hollow fiber membrane. The effect of starch type, starch concentration, temperature, pH, reaction time and agitation rate on the CD production were determined by using one factor at a time (OFAT). The results revealed that 3% (w/v) of soluble potato starch produced the highest amount of CD (5.22 mg/mL). The production of CD was also the highest at temperature of 40°C (5.21 mg/mL), pH 6 (4.62 mg/mL), 6 h of reaction time (4.37 mg/mL) and 150 rpm of agitation rate (4.38 mg/ml). From the result, immobilized CGTase managed to produce 2 to 3-fold higher of CD production than the free CGTase. The screening of the significant reaction parameters for the production of CD were conducted by using fractional factorial design (FFD). The results showed that the concentration of soluble potato starch concentration, pH and temperature were the most significant parameters on the production of CD. The reaction parameters were then optimized by using central composite design (CCD). Under the optimized conditions (3.2% w/v concentration of soluble potato starch, 45.2°C of reaction temperature and pH 5.6), the production of CD was 3.2-fold higher than the amount of CD before the optimization process. The immobilized enzyme was successfully retained up to 38% of the initial activity and can be reused for 10 cycles for the production of CD. Therefore, the immobilization of CGTase on hollow fiber membrane proved to be suitable to enhance the production of CD with high enzyme stability.