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Optimization and kinetic study of pigments production on solid state fermentation using oil palm frond by monascus purpureus ftc 5357 / Nur Fathin Shamirah Daud

By:

Nur Fathin Shamirah Daud [author.]

Material type: Text

text
text

Media type:

unmediated
computer

Carrier type:

volume
computer disc

ISBN:

THE0009184(Local)

Subject(s):

Dissertation note: Thesis (Master of Science) -- Universiti Malaysia Pahang – 2021 Abstract: Monascus sp. produce useful Monascus pigments that widely used in food industry. Extensive study available on Monascus sp. in solid-state fermentation (SSF) using shake flask, however, cultivation of Monascus sp. in stirred drum bioreactor is insufficiently reported. Thus, this study aims to optimize pigments production (i.e. yellow, orange and red) from Monascus purpureus FTC 5357 using oil palm frond (OPF) in 5 L stirred-drum bioreactor. Prior to the SSF process, hydrothermal treatment (121 °C, 15 min) was done. A three-level Box–Behnken design (BBD) was used for the optimization, with threeparameters such as initial moisture content (IMC), aeration rate (AR) and peptone concentration (PC). The dried fermented OPF were extracted and analysed for pigments production (i.e. yellow, orange and red), biomass and glucose concentration. Next, the kinetic profile of the optimize condition and kinetic parameters were also been evaluated. Based on the ANOVA analysis results, IMC, AR and PC contributed significantly to the pigments production. The optimal fermentation conditions resulted IMC at 70 % w/w, AR at 1.30 vvm and PC at 4.40 % w/w. Under these conditions, the pigments production were obtained at 22.03, 20.11 and 18.59 Au/g.d for yellow, orange and red, respectively. Validation experiment was done to confirm the adequacy of the models. The fermentation profile at optimized condition indicated that Monascus purpureus FTC 5357 reached maximum pigments and biomass at day 9. Results obtained from this study showed the importance of IMC, AR and PC for pigments production and biomass. Meanwhile, the production kinetic parameters such as maximum specific growth rate, the growth yield, pigment yield, cell productivity, pigments productivity for yellow, orange and red were 0.707 day-1, 0.15 mg/mg, 0.193 Au/mg cell and 12.514 mg/(g.day), 2.447 Au/(g.d.day), 2.234 Au/(g.d.day) and 2.065 Au/(g.d.day), respectively. The pigments produced through SSF process using OPF as a substrate by Monascus purpureus FTC 5357 has a great potential to be utilized as a source of pigment for food in future.

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Holdings ( 2 )
Title notes ( 4 )

Holdings
Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode
Thesis	UMPLIB GAMBANG Reference	Reference	CD13042 (Browse shelf(Opens below))	1	Not for loan		T000001750
Thesis	UMPLIB GAMBANG Reference	Reference	FTKKP .F38 2021 r Thesis (Browse shelf(Opens below))	1	Not for loan		T000001749

Faculty of Chemical and Process Engineering Technology

Thesis (Master of Science) -- Universiti Malaysia Pahang – 2021

Includes bibliographical references

Monascus sp. produce useful Monascus pigments that widely used in food industry. Extensive study available on Monascus sp. in solid-state fermentation (SSF) using shake flask, however, cultivation of Monascus sp. in stirred drum bioreactor is insufficiently reported. Thus, this study aims to optimize pigments production (i.e. yellow, orange and red) from Monascus purpureus FTC 5357 using oil palm frond (OPF) in 5 L stirred-drum bioreactor. Prior to the SSF process, hydrothermal treatment (121 °C, 15 min) was done. A three-level Box–Behnken design (BBD) was used for the optimization, with threeparameters such as initial moisture content (IMC), aeration rate (AR) and peptone concentration (PC). The dried fermented OPF were extracted and analysed for pigments production (i.e. yellow, orange and red), biomass and glucose concentration. Next, the kinetic profile of the optimize condition and kinetic parameters were also been evaluated. Based on the ANOVA analysis results, IMC, AR and PC contributed significantly to the pigments production. The optimal fermentation conditions resulted IMC at 70 % w/w, AR at 1.30 vvm and PC at 4.40 % w/w. Under these conditions, the pigments production were obtained at 22.03, 20.11 and 18.59 Au/g.d for yellow, orange and red, respectively. Validation experiment was done to confirm the adequacy of the models. The fermentation profile at optimized condition indicated that Monascus purpureus FTC 5357 reached maximum pigments and biomass at day 9. Results obtained from this study showed the importance of IMC, AR and PC for pigments production and biomass. Meanwhile, the production kinetic parameters such as maximum specific growth rate, the growth yield, pigment yield, cell productivity, pigments productivity for yellow, orange and red were 0.707 day-1, 0.15 mg/mg, 0.193 Au/mg cell and 12.514 mg/(g.day), 2.447 Au/(g.d.day), 2.234 Au/(g.d.day) and 2.065 Au/(g.d.day), respectively. The pigments produced through SSF process using OPF as a substrate by Monascus purpureus FTC 5357 has a great potential to be utilized as a source of pigment for food in future.