TY  - MANSCPT
AU  - Aliyah Binti Jamaludin
TI  - Study on the application of greensynthesized silver nanoparticles using sago (metroxylon sagu) as an antibacterial agent in enzymatic biofuel cell
SN  - THE0009825 (Local)
PY  - 2023///
CY  - Kuantan, Pahang
PB  - UMPSA
KW  - Faculty of Chemical and Process Engineering Technology
KW  - Dissertations
KW  - Universities and colleges
N1  - Faculty of Chemical and Process Engineering Technology; Thesis (Master of Science) -- Universiti Malaysia Pahang – 2023; Hydrogen energy is among the cleanest sources of alternative energy, and a fuel cell produces electricity from it most effectively. Moreover, an enzymatic biofuel cell (EBFC) is a fuel cell type that uses enzymes to replace the use of metallic inorganic catalysts, as used in different fuel cell types. Sugar is the most commonly used biofuel in EBFC. In this work, direct conversion of energy from sago (Metroxylon sagu) in EBFC was performed to simplify the fabrication. However, biofuels based on natural polymers face the problem of being easily attacked by microorganisms, which can deteriorate the fuel cell's total performance. In order to solve this issue, silver nanoparticles (AgNPs) are added to EBFC in this research as an antibacterial agent. AgNPs have traditionally been produced through a number of complex stages that demand close attention. This research showed a simpler autoclaving green synthesis method for producing AgNPs in a single pot. Sago has once more been employed in the green synthesis of AgNPs as a reducing, capping, as well as stabilizing agent. The average particle size of the AgNPs is 17.2 ± 6.7 nm, and they are spherical and evenly distributed. Against gram-positive (M.luteus) and gram-negative (E.coli) bacteria, the AgNPs also portrayed strong antibacterial activities. Prior to using the AgNPs as an antibacterial agent, response surface methodology (RSM) was used to optimize the fabrication of EBFC. Then, the factors influencing the performance of EBFC were priorly examined using the one-factor-at-a-time (OFAT) method to obtain the optimum range of parameters. The parameters considered in the optimization were substrate loading, enzyme volume, working temperature, and pH to achieve the best electrochemical performances in terms of maximum power density (MPD), open circuit voltage (OCV), anodic current (AC), and cathodic current (CC). Under the best condition, the EBFC achieved the best performance of MPD at 39.58 μW cm-2, OCV at 0.35 V, AC, and CC at 48.33 μA and 48.21 μA, respectively. Finally, the green synthesized AgNPs were applied in the best condition of EBFC as an antibacterial agent. The antibacterial activities and electrochemical performances were investigated by comparing the EBFCs with and without the addition of AgNPs. AgNPs were discovered to be a good antibacterial agent against S.aureus and E.coli, extending the EBFC's lifespan by more than 10-fold. In addition, the AgNPs also enhanced the electrochemical performances in terms of MPD and OCV. MPD was maintained for less than 15% deterioration, and OCV increased to 65% within the same 24 h storage time. This study proves that the employment of AgNPs as an antibacterial agent in EBFC not only inhibits the growth of bacteria but also improves electrochemical performances
ER  -