04118ntm a2200337 i 4500 MY-KuUP 20251223182547.0 t||||fr|||| 000 0 ta 251223t20252025my a|||fr|||| 000 0 eng d THE0010176 (Local) Hardback UMPSA eng UMPSA rda FTKKP .J83 2025 r Thesis Juanni Zhang, author. Fabrication of biocompatible chitin-derived dual-network nanocomposite hydrogels with antibacterial and blood coagulation properties / Juanni Zhang Kuantan Pahang : UMPSA 2025 © 2025 xvii, 252 pages : illustrations ; 30 cm. + 1 CD-ROM rdacontent text rdamedia unmediated rdacarrier volume rda text file PDF Faculty of Chemical and Process Engineering Technology Thesis (Doctor of Philosophy) -- Universiti Malaysia Pahang – 2025 Includes bibliographical references Chitin, the second most abundant natural polysaccharide after cellulose, is a renewable biopolymer composed of β-(1→4)-linked N-acetylglucosamine units. Despite its excellent biocompatibility, degradability, and modifiability, the practical application of chitin remains limited by its poor solubility. Chemical modification can significantly enhance chitin's solubility and functionality, facilitating the research and development of chitin-derived hydrogels. However, chitin-derived hydrogels still encounter challenges such as insufficient mechanical strength, poor biocompatibility, and limited antibacterial properties. To address the limitations, this study integrates dual-network and nanocomposite technologies to develop chitosan/HAp@Zn dual-network nanocomposite hydrogels and quaternized chitosan/chitin nanowhisker dual-network nanocomposite hydrogels, aiming to enhance their properties and broaden their potential applications. The former comprises chitosan, zinc-loaded nano-hydroxyapatite (HAp@Zn), acrylylglycine (ACG), and N-benzylacrylamide (NBAA), while the latter consists of quaternized chitosan (QCS), chitin nanowhiskers (ChWs), acrylic acid, acrylamide, and Zn²+. The influence of the concentration of each component on the properties of the hydrogel was investigated by one-factor-at-a-time analysis, and the structure of the hydrogel was characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and scanning electron microscopy (SEM). Additionally, the physical properties (mechanical strength, swelling property, rheological behavior) and biological properties (antibacterial, cytotoxicity, hemolysis, blood coagulation) were systematically examined. The results indicate that the chitosan/HAp@Zn hydrogel possesses excellent mechanical strength and pH-responsive swelling performance. Its maximum mechanical strength is 495.2 kPa and its equilibrium swelling rate is 23.4g/g. The maximum antibacterial rates of E. coli and S. aureus reached 100% and 99.9% respectively. These hydrogels are not cytotoxic and can demonstrate excellent blood compatibility by adjusting their composition. Additionally, they were found to have a good ability for blood coagulation. For the QCS/ChWs hydrogels, the maximum mechanical strength and equilibrium swelling rate are 391.9 kPa and 59.0 g/g, respectively. The maximum antibacterial rates of E. coli and S. aureus were as high as 99.7%. Furthermore, they exhibit superior blood compatibility and no cytotoxicity as well. Most importantly, they show the ability to clot blood rapidly. This study significantly improves the comprehensive properties of chitin-derived hydrogels. It provides an important theoretical basis and technical support for its application in tissue engineering, antibacterial, and hemostatic materials. Faculty of Chemical and Process Engineering Technology Dissertations Universities and colleges Dissertations Theses Dissertations lcc THESIS 0 0 lcc 0 0 10000 10000 2025-12-23 0 FTKKP .J83 2025 r Thesis T000003943 2025-12-23 18:26:13 2025-12-23 RESTRICT 0 0 lcc 0 6 10000 10000 2025-12-23 0 CD13842 T000003944 2025-12-23 18:26:47 2025-12-23 RESTRICT 103795 103801