03001nam a22003137a 4500003000800000005001700008006001900025007000300044008004100047020003300088040002700121100003300148245015600181264003600337264001200373300003700385336002100422337002500443338002300468347002400491500005000515502013600565504003800701520175700739610006502496650004502561650003702606856004402643MY-KuUP20260121152804.0t||||fs|||| 000 0 ta260121t20252025my a|||fs|||| 000 0 eng d  aTHE0010295 (Local)qHardback  aUMPSAbengcUMPSAerda1 aYun Ning, Grace Ng,eauthor.10aEffect of murashige and skoog  medium strength and plant growth  regulators on the growth of  momordica cochinchinesis (gac fruit) /cGrace Ng Yun Ning 1aKuantan, Pahang :bUMPSA,c2025 4c© 2025  axiv, 86 pages :billustrations ;  2rdacontentatext  2rdamediaaunmediated  2rdacarrieravolume  2rdaatext filebPDF  aFaculty of Industrial Sciences and Technology  aFinal Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025  aInclude bibliographical reference3 aMomordica cochinchinensis, or Gac fruit, is a tropical superfruit known for its high  nutritional and medicinal value but faces cultivation challenges such as low seed viability,  poor germination, and environmental stress. Tissue culture offers a solution by enabling  controlled propagation of elite plants. This study aimed to improve sterilization and  optimize growth media for better in vitro propagation. Sodium hypochlorite  concentrations for surface sterilization, Murashige and Skoog medium strengths, and  cytokinin concentrations were evaluated for their role in affecting shoot growth and  proliferation. Best surface sterilization was under 15% NaOCl for 10 minutes where 0%  contamination rate was achieved, ensuring aseptic explant preparation. Among the tested  MS medium strengths, full, half, and quarter, full-strength MS provided the best results,  with the highest number of shoots (6.33 ± 0.33), shoot length (1.80 ± 0.06 cm), and leaf  formation (4.67 ± 0.67) after five weeks. The study also tested various BAP  concentrations (0, 1.0, 2.0, 3.0, and 4.0 mg/L), with 4.0 mg/L showing the highest shoot  proliferation (16.67 ± 0.88), shoot length (4.50 ± 0.17 cm), and leaf number (12.00 ±  1.15), emphasizing the importance of hormonal optimization. The results highlight the  significance of optimizing sterilization methods and media formulations to improve Gac  fruit tissue culture efficiency, providing a foundation for sustainable cultivation and  commercial production. Statistical analysis using One-Way ANOVA (p ≤ 0.05) showed  that MS strength significantly affected all growth parameters, while BAP concentration  significantly influenced shoot length but had no significant effect on shoot and leaf  numbers20aFaculty of Industrial Sciences and TechnologyxDissertations 0aUniversities and collegesxDissertations 0aFinal Year ReportxDissertations  uhttp://umpir.ump.edu.my/id/eprint/45601