03289ntm a2200301 i 4500003000800000005001700008006001900025007000300044008004100047020003300088040002700121100002600148245012400174264003600298264001200334300003800346336002100384337002500405338002300430347002400453500005000477502013600527504003800663520213900701610006502840650004502905650003702950MY-KuUP20260121173950.0t||||fs|||| 000 0 ta260121t20252025my a|||fs|||| 000 0 eng d  aTHE0010300 (Local)qHardback  aUMPSAbengcUMPSAerda1 aLau, En Lin,eauthor.10aInvestigating the role of  complement c3 and c3a in human  type 1 diabetes mellitus patients in  Malaysia /cLau En Lin 1aKuantan, Pahang :bUMPSA,c2025 4c© 2025  axiii, 77 pages :billustrations ;  2rdacontentatext  2rdamediaaunmediated  2rdacarrieravolume  2rdaatext filebPDF  aFaculty of Industrial Sciences and Technology  aFinal Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025  aInclude bibliographical reference3 aThe incidence of Type 1 diabetes mellitus (T1DM) has increased globally over the years.  Accumulating research suggests that the human complement system may contribute to  the pathogenesis of diabetes and induce vascular complications that characterize this  condition. Several discoveries from countries such as the UK, China and United States  indicated a significant level of complement C3 and C3a in T1DM patients compared to  healthy individuals. However, no relevant study has been conducted among T1DM  patientsin Malaysia. This study investigates the role of complement C3 and C3a in T1DM  patients in Malaysia, with the association of phenotypic characteristics such as age  group, ethnicity and gender. A total of 18 (9 T1DM patients and 9 health individuals)  were involved in this study and blood serum was obtained for both complement C3 and  C3a analysis. The human complement C3 sandwich ELISA kit and human complement  C3a sandwich ELISA kit were employed to determine both complement C3 and C3a  concentrations in blood serum. The Wilcoxon matched-pairs signed rank test with a Pvalue, 0.1641 for complement C3 and P-value > 0.9999 for complement C3a do not  demonstrate any appreciable differences among T1DM patient and healthy control.  Based on the findings, the complement C3 and C3a level do not associated with  phenotypic characteristics (age, gender and ethnicity) among T1DM patients and healthy  controls. As evidence, both Wilcoxon matched-pairs signed rank test (gender) and  Kruskal-Wallis tests (age group and ethnicity) were run among T1DM patients and  healthy control. P-values obtained (0.3828, 0.1070 and 0.1341) for complement C3  analysis among this group do not show significant differences with these phenotypic  characteristics. The results go same with the complement C3a analysis with a P-value of  0.7422 for gender, 0.5283 for age group, and 0.6769 for ethnicity, demonstrating no  statistically significant variations based on these phenotypic factors. Therefore,  complement C3 and C3a may not be a good indicator in distinguishing between T1DM  patient and healthy control in Malaysia.20aFaculty of Industrial Sciences and TechnologyxDissertations 0aUniversities and collegesxDissertations 0aFinal Year ReportxDissertations