02686nrm a2200301 i 4500003000800000005001700008006001900025007000300044008004100047020003300088040002700121100002800148245015500176264003600331264001200367300003700379336002100416337002500437338002300462347002400485500005000509502013600559504003800695520150400733610006502237650004502302650003702347MY-KuUP20260123124833.0t||||fr|||| 000 0 ta260123t20252025my ||| f |||| s0| 0 eng d  aTHE0010305 (Local)qHardback  aUMPSAbengcUMPSAerda1 aLim, Kok Ling,eauthor.10aEvaluation of antibacterial properties and topical application  of kyllinga nemoralis extract in  allergic-induced skin lesion in rats /cLim Kok Ling 1aKuantan, Pahang :bUMPSA,c2025 4c© 2025  axv, 106 pages :billustrations ;  2rdacontentatext  2rdamediaaunmediated  2rdacarrieravolume  2rdaatext filebPDF  aFaculty of Industrial Sciences and Technology  aFinal Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025  aInclude bibliographical reference3 aTraditional medicine as a vital source of healthcare globally, with plants like Kyllinga  nemoralis offering significant therapeutic potential. Despite its extensive ethnomedicinal  use, the antibacterial efficacy of K. nemoralis, particularly in managing skin allergies  complicated by bacterial infections, has not been extensively studied. This study aimed  to investigate the antibacterial activity of K. nemoralis and its therapeutic effects in an  allergy-induced rat model. To achieve this goal, K. nemoralis methanolic extract was  prepared using Soxhlet apparatus, followed by phytochemical analysis via Gas  Chromatography-Mass Spectrometry (GC-MS). Antibacterial activity of K. nemoralis was assessed through agar disc diffusion and minimum inhibitory concentration (MIC)  assays against Bacillus subtilis and Escherichia coli. A 10% K. nemoralis extract cream  was formulated and tested in an-allergy-induced dermatitis rat model, which established  using 2,4-dinitrochlorobenzene (DNCB). The extract demonstrated moderate antibacterial activity, with MIC values of 1.88 mg/mL against B. subtilis and 3.75 mg/mL  against E. coli. In vivo, the 10% extract cream significantly reduced dermatitis scores of  the treatment group from 6.60 to 1.80, indicating its efficacy in combating bacterial  infections and alleviating allergic skin conditions. These findings highlighted K.  nemoralis as a natural antibacterial agent and a therapeutic option for managing skin  infections and allergies20aFaculty of Industrial Sciences and TechnologyxDissertations 0aUniversities and collegesxDissertations 0aFinal Year ReportxDissertations