Nur Shahira Binti Razali, <br/><br/>
Molecular identification of a fungi isolate from liquid culture of colon cancer biopsy / 
Nur Shahira Binti Razali 
 - xiii, 64 pages :  illustrations ; 
<br/><br/> Faculty of Industrial Sciences and Technology 
<br/><br/>Final Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025 
<br/><br/>Include bibliographical reference 
<br/><br/> Fungal infections are increasingly recognized as significant factors affecting various  health issues in humans, including cancer. This research aimed to explore fungal species  obtained from liquid cultures derived from colon cancer biopsy samples, particularly  seeking to identify possible connections between fungal presence and colon cancer  pathology. Liquid cultures from biopsies were carefully prepared, and fungal isolates  were grown under regulated laboratory conditions. Morphological traits and growth  behaviors were documented to ensure precise differentiation of the isolates. Molecular  classification of the fungi was conducted utilizing Polymerase Chain Reaction (PCR)- based methods that target specific genetic markers of fungi. DNA sequencing and  phylogenetic analysis additionally validated the taxonomic identification of the isolates.  The findings indicated the presence of Gymnopilus sp. within the biopsy cultures, with  some isolates exhibiting distinct genetic variations. Initial assessments imply possible  interactions between the identified fungi and the tumor microenvironment, warranting  further study. This research emphasizes the significance of investigating fungal  microbiota in cancer studies and highlights the critical need for molecular identification  techniques to uncover potential microbial contributors to cancer development. Future  research will aim to clarify the mechanistic roles of identified fungal species in the  progression of colon cancer, providing insights into innovative diagnostic and therapeutic  strategies. 
<br/><br/><label>ISBN: </label>THE0010315 (Local) 
<br/><br/><label>Subjects--Corporate Names: </label><br/>Faculty of Industrial Sciences and Technology--Dissertations
<br/><br/><label>Subjects--Topical Terms: </label><br/>Universities and colleges--Dissertations<br/>Final Year Report--Dissertations