02764nam a2200301 i 4500003000800000005001700008006001900025007000300044008004100047020003300088040002700121100004200148245012500190264003600315264001200351300003700363336002100400337002500421338002300446347002400469500005000493502013600543504003800679520159800717610006502315650004502380650003702425MY-KuUP20260227150219.0t||||fr|||| 000 0 ta260227t20252025my a|||fr|||| 000 0 eng d  aTHE0010402 (Local)qHardback  aUMPSAbengcUMPSAerda1 aAieda Farihin Binti Bakhary,eauthor.10aEvaluating the antifungal effects  of crude protease extracted from  lactic acid bacteria /cAieda Farihin Binti Bakhary 1aKuantan, Pahang :bUMPSA,c2025 4c© 2025  axii, 95 pages :billustrations ;  2rdacontentatext  2rdamediaaunmediated  2rdacarrieravolume  2rdaatext filebPDF  aFaculty of Industrial Sciences and Technology  aFinal Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025  aInclude bibliographical reference3 aCandida species, particularly Candida albicans, are the primary causes of invasive  candidiasis. The scarcity of therapeutic choices and the growing frequency of antifungal  resistance highlight the critical need for new treatments. This study aimed to investigate  the antifungal activity of lactic acid bacteria (LAB) isolates obtained from pickled  maman, Cleome gynandra. A total of 10 LAB isolates were screened using a dual agar  overlay assay to evaluate their antifungal activity based on zones of inhibition against C.  albicans. The C. albicans used was confirmed via Germ Tube Test. All 10 isolates  showed antifungal activity against C. albicans, ranging from weak to strong inhibition.  Three isolates (M5, M6, M9) produced the largest zones of inhibition with 17 ± 1.73 mm,  14 ± 0.57 and 18 ± 0.57 mm, respectively. These LAB isolates were further analyzed for  protease enzyme activity using the Lowry method on their cell-free supernatants. All  three LAB isolates were shown to produce protease activity. M6 had the highest activity  (1.212 × 10-5 U/mL), followed by M5 (8.4 × 10-6 U/mL) and M9 (4.7 × 10-6 U/mL). These  results demonstrated that specific LAB isolates possess significant antifungal activity and  their abilities to produce protease enzymes. These findings are preliminary report  suggesting LAB isolates from pickled maman shows promising potential as natural  therapeutic agents against resistant strains of Candida. Further research is warranted to  unravel their antifungal mechanisms and assess their viability in clinical and industrial  settings20aFaculty of Industrial Sciences and TechnologyxDissertations 0aUniversities and collegesxDissertations 0aFinal Year ReportxDissertations