02860nam a2200337 i 4500 MY-KuUP 20260227150219.0 t||||fr|||| 000 0 ta 260227t20252025my a|||fr|||| 000 0 eng d THE0010402 (Local) Hardback UMPSA eng UMPSA rda FIST .A34 2025 r Bc. Aieda Farihin Binti Bakhary, author. Evaluating the antifungal effects of crude protease extracted from lactic acid bacteria / Aieda Farihin Binti Bakhary Kuantan, Pahang : UMPSA, 2025 © 2025 xii, 95 pages : illustrations ; rdacontent text rdamedia unmediated rdacarrier volume rda text file PDF Faculty of Industrial Sciences and Technology Final Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025 Include bibliographical reference Candida species, particularly Candida albicans, are the primary causes of invasive candidiasis. The scarcity of therapeutic choices and the growing frequency of antifungal resistance highlight the critical need for new treatments. This study aimed to investigate the antifungal activity of lactic acid bacteria (LAB) isolates obtained from pickled maman, Cleome gynandra. A total of 10 LAB isolates were screened using a dual agar overlay assay to evaluate their antifungal activity based on zones of inhibition against C. albicans. The C. albicans used was confirmed via Germ Tube Test. All 10 isolates showed antifungal activity against C. albicans, ranging from weak to strong inhibition. Three isolates (M5, M6, M9) produced the largest zones of inhibition with 17 ± 1.73 mm, 14 ± 0.57 and 18 ± 0.57 mm, respectively. These LAB isolates were further analyzed for protease enzyme activity using the Lowry method on their cell-free supernatants. All three LAB isolates were shown to produce protease activity. M6 had the highest activity (1.212 × 10-5 U/mL), followed by M5 (8.4 × 10-6 U/mL) and M9 (4.7 × 10-6 U/mL). These results demonstrated that specific LAB isolates possess significant antifungal activity and their abilities to produce protease enzymes. These findings are preliminary report suggesting LAB isolates from pickled maman shows promising potential as natural therapeutic agents against resistant strains of Candida. Further research is warranted to unravel their antifungal mechanisms and assess their viability in clinical and industrial settings Faculty of Industrial Sciences and Technology Dissertations Universities and colleges Dissertations Final Year Report Dissertations lcc THESIS 0 0 lcc 0 1 0 10000 10000 2026-02-27 0 FIST .A34 2025 r Bc. T000003717 2026-02-27 15:02:59 2026-02-27 RESTRICT 104253 104259