03064ntm a2200349 i 4500 MY-KuUP 20260302162631.0 t||||fs|||| 000 0 c| |||aaa||||a 260302t20252025my a|||fs|||| 000 0 eng d THE0010409 (Local) Hardback UMPSA eng UMPSA rda FIST .H86 2025 r Bc. Mathumittha Tamil Selvan, author. The formulation and effect of tiger milk mushroom and cordyceps from hot water extraction method for therapeutic applications / Mathumittha Tamil Selvan Kuantan, Pahang : UMPSA, 2025 © 2025 xiii, 70 pages : illustrations ; rdacontent text rdamedia unmediated rdacarrier volume rda text file PDF Faculty of Industrial Sciences and Technology Final Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025 Include bibliographical reference This study explores the therapeutic potential of tiger milk mushroom (Lignosus rhinocerus) and cordyceps (Cordyceps sinensis) extracts, focusing on their antiinflammatory and antioxidant properties when formulated in different ratios. Using hot water extraction, the study prepared five formulations, including pure extracts of each mushroom and combinations in ratios of 1:1, 1:2, and 2:1. These formulations were evaluated for bioactivity through assays such as Bovine Serum Albumin (BSA) denaturation for anti-inflammatory properties and DPPH radical scavenging and FRAP assays for antioxidant potential. The findings revealed that all formulations displayed significant bioactivity, with the 2:1 ratio of tiger milk mushroom to cordyceps (Formulation 5) demonstrating the highest efficacy in both its anti-inflammatory and antioxidant properties, highlighting a synergistic interaction when compared to the 1:1 and 1:2 ratios. Chemical analysis using FTIR and HPLC confirmed the presence of bioactive compounds, including phenolic acids, and other antioxidant agents, which are key contributors to the observed effects. The study underscores the synergistic potential of these mushrooms in combating oxidative stress and inflammation-related diseases, presenting a natural, low-side-effect alternative to conventional therapies. The study paves the way for the development of safe and effective natural formulations as alternatives to conventional drugs with fewer side effects. Moreover, these results offer a foundation for future research, including in vivo studies and large-scale production, to further explore and optimize these natural formulations for therapeutic use. Faculty of Industrial Sciences and Technology Dissertations Universities and colleges Dissertations Final Year Report Dissertations http://umpir.ump.edu.my/id/eprint/45757 lcc THESIS 0 0 lcc 0 1 0 10000 10000 2026-03-02 0 FIST .H86 2025 r Bc. T000003724 2026-03-02 16:26:53 2026-03-02 THESIS 104260 104266