Marina Airin Binti Othman , , creator author. text my 2025 2025 monographic eng 54 pages : illustrations ; The purpose of this research is to examine the adsorption kinetics, isotherms, and thermodynamics associated with the immobilization of cyclodextrin glucanotransferase (CGTase) on pineapple peel. Because of their encapsulating qualities, cyclodextrins (CD), which are produced by the enzyme CGTase, find widespread use in the food and pharmaceutical industries. Because of its chemical stability and sustainability, pineapple peel which is a plentiful agricultural waste was selected as the support material. A range of parameters, such as enzyme concentrations (10,30,50,70,90 U), contact durations (10,15,20,25,30 hours), and temperatures (15, 20, 25, 30, 35 °C), were used to study the adsorption process. The adsorption behavior was described using three adsorption isotherm models: Langmuir, Freundlich, and Temkin. The adsorption kinetics were studied using Pseudo-first order (PFO), Pseudo-second order (PSO), and Elovich models. Parameters of thermodynamics like Gibbs free energy (ΔG) were also examined in order to gauge the process's spontaneity. The enzyme activity of 22.48 U/mL, 30 U, 25oC and 25 h was found to be the ideal concentration, temperature and contact time. The findings demonstrated monolayer adsorption by showing that CGTase immobilization on pineapple peel adhered to the Langmuir isotherm model. The PSO model indicated chemisorption as the predominant process and provided the best description of the adsorption kinetics. The spontaneity of the process was confirmed by thermodynamic analysis, which showed negative ΔG values of -3.44 kJ/mol. High immobilization yield and activity retention were the outcomes of immobilization settings that were ideal, which were 20 °C, 25 hours of contact time, and 30 U of enzyme concentration. According to this study, pineapple peel is an economical and environmentally beneficial support material for enzyme immobilization, which helps with waste valuation and long-term industrial uses. specialized Marina Airin Binti Othman Faculty of Chemical and Process Engineering Technology Final Year Report (Bachelor of Chemical Engineering ) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025 Include bibliographical reference Faculty of Chemical and Process Engineering Technology Dissertations Universities and colleges Dissertations Theses Dissertations THE0010686 (Local) https://umpir.ump.edu.my/id/eprint/47498 https://umpir.ump.edu.my/id/eprint/47498 UMPSA 260709 20260709094953.0 eng