03315ntm a2200349 i 4500 MY-KuUP 20260710121017.0 t|||||r|||| 000 0 ta 260710t20252025my a|||fr|||| 000 0 eng d THE0010703 (Local) Hardback UMPSA eng UMPSA rda FTKKP .I57 2025 r Bc. Nurin Rasyiqah Binti Mohd Rashdan, , , author. Study on the enzyme kinetics of xylanase in poultry feed hydrolysis / Nurin Rasyiqah Binti Mohd Rashdan Kuantan, Pahang : UMPSA, 2025 © 2025 xv, 46 pages : illustrations ; rdacontent text rdamedia unmediated rdacarrier volume rda text file PDF Faculty of Chemical and Process Engineering Technology Final Year Report (Bachelor of Chemical Engineering ) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025 Include bibliographical reference Poultry is a common source of protein that is essential for our daily sustenance. Worldwide poultry production has expanded in response to rising demand for poultry meat and eggs due to population, urbanization and industrialization. Poultry lacks the digestive enzymes necessary to fully digest fiber. Hence, they require commercial exogenous enzymes in their diets to aid digestion. Adding enzymes to poultry feed enhances enzyme-substrate binding efficiency, thereby improving the formulation of commercial poultry feed. The main objective of this research is to investigate the enzyme kinetics of commercial xylanase in poultry feed hydrolysis. The experiments were conducted with varying substrate concentrations (2%, 4%, 6%, 8%, and 10% (w/v)) and pH (4, 5, 6, 7 and 8) at a 120 minutes of reaction time. It is done by reacting poultry feed with xylanase enzyme. The enzymatic reaction was then stopped by adding dinitrosalicylic acid (DNS) reagent. The absorbance was read using a UV-Vis spectrophotometer. Further analysis for optimization of enzyme activity using Central Composite Design (CCD) and the kinetic parameters (Km and Vmax) were analyzed using the Michaelis-Menten method. The best results were found at 8% (w/v) substrate concentration and the pH 6 which the highest xylanase activity was at 1.0829 U/ml (xylan corn cob) and 1.9370 U/ml (poultry feed). At pH 6, xylan corn cob shows a higher Vmax (1.0536 mol min-1 mg-1) and lower Km (0.8150 mg/ml) compared to poultry feed (Vmax = 0.4294 μmol min-1 mg-1, Km = 9.0081 mg/ml), indicating greater catalytic efficiency and substrate affinity. In conclusion, understanding enzyme reaction rate and substrate interactions can help increase the degradation of complex non-starch polysaccharides (NSPs) and improve nutrient release and utilization in poultry. This, in turn, can help minimize the integration of costly feed supplements into poultry feed formulation, thus providing a cost-effective and sustainable poultry feed formulation. Faculty of Chemical and Process Engineering Technology Dissertations Universities and colleges Dissertations Theses Dissertations https://umpir.ump.edu.my/id/eprint/47568 lcc PSM 0 0 lcc 0 0 10000 10000 2026-07-10 0 FTKKP .I57 2025 r Bc. T000004297 2026-07-10 12:10:38 2026-07-10 PSM 105170 105176