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  <titleInfo>
    <title>Molecular biomethods handbook</title>
  </titleInfo>
  <name type="personal">
    <namePart>Rapley, Ralph</namePart>
  </name>
  <name type="personal">
    <namePart>Walker, John M.</namePart>
  </name>
  <typeOfResource>text</typeOfResource>
  <genre authority="marc">bibliography</genre>
  <originInfo>
    <place>
      <placeTerm type="code" authority="marccountry">nju</placeTerm>
    </place>
    <place>
      <placeTerm type="text">Totowa, NJ</placeTerm>
    </place>
    <publisher>Humana Press</publisher>
    <dateIssued>c2008</dateIssued>
    <dateIssued encoding="marc">2009</dateIssued>
    <edition>2nd ed.</edition>
    <issuance>monographic</issuance>
  </originInfo>
  <language>
    <languageTerm authority="iso639-2b" type="code">eng</languageTerm>
  </language>
  <physicalDescription>
    <form authority="marcform">print</form>
    <extent>xx, 1124 p. : ill. ; 26 cm.</extent>
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  <tableOfContents>The manipulation of nucleic acids : basic tools and techniques / Gayle Corkill and Ralph Rapley -- Restriction enzymes : tools in clinical research / Gareth J.S. Jenkins -- Principles and medical applications of the polymerase chain reaction / Bimal D.M. Theophilus -- Probe design, production, and applications / Marilena Aquino de Muro -- Southern blotting as a diagnostic method / Bronwen M. Harvey and Pirkko Soundy -- Capillary electrophoresis of DNA : biomedical applications / Beatriz Sanchez-Vega -- Denaturing high-performance liquid chromatography (DHPLC) for nucleic acid analysis / Kim Hung Leung and Shea Ping Yip -- Denaturing gradient gel electrophoresis (DGGE) / Jeroen H. Roelfsema and Dorien J.M. Peters -- Single strand conformation polymorphism (SSCP) analysis / Kim Hung Leung and Shea Ping Yip -- Randomly amplified polymorphic DNA (RAPD) : a useful tool for genomic characterization of different organisms / Lúcia Maria da Cunha Galvão and Eliane Lages-Silva -- Quantification of mRNA using real time RT-PCR : the SYBR solutiion / David Sugden and Patricia de Winter -- Quantitative analysis of DNA sequences by PCR and solid-phase minisequencing / Anu Suomalainen and Ann-Christine Syvänen -- Multiplex amplifiable probe hybridization (MAPH) / Carolina Sismani, Ludmila Kousoulidou, and Philippos C. Patsalis -- Gene expression profiling / Arnis Druka, Robbie Waugh, and Pete Hedley -- Comparative genomic hybridization in clinical and medical research / Peng-Hui Wang, Yann-Jang Chen, and Chi-Hung Lin --  Subtractive hybridization / Craig Winstanley -- Fluorescence in situ hybridization / Jane Bayani and Jeremy A. Squire -- Quantitative trait locus mapping to identify genes for complex traits in mice / Jonathan D. Smith -- cDNA microarrays / Phillip G. Febbo -- Mapping techniques / Simon G. Gregory -- Single nucleotide polymorphisms / Nameeta Shah -- Gene/protein sequence analysis : a compilation of bioinformatic tools / Bernd H.A. Rehm and Frank Reinecke -- Protein electrophoresis / David Sheehan and Siobhan O'Sullivan -- Protein blotting / Patricia Gravel -- Capillary electrophoresis of proteins / Mark Strege -- Autoradiograph and fluorography / Bronwen M. Harvey -- Mass spectrometry of proteins and peptides / Kenneth G. Standing -- Post-translational modifications of proteins / Christoph Kannich and Birte Fuchs -- Protein microarray technology / Charlotte  H. Clarke and Eric T. Fung -- Protein-protein interactions / Hae Ryoun Park ... [et al.] -- Glycoprotein analysis / Terry D. Butters and David C.A. Neville -- Solid-phase peptide synthesis / Mare Cudic and Gregg B. Fields -- Monoclonal antibodies / Zhong J. Zhang and Maher Albitar -- Antibody phage display / Rob Aitken -- Protein engineering / Thomas Willemsen ... [et al.] -- Directed protein evolution / Sabine C. Stebel ... [et al.] -- Enzyme linked immunosorbent assay (ELISA) / John Crowther -- Epitope mapping : identification of antibody-binding sites on protein antigens / Glenn E. Morris -- Quantum dots / Charles Z. Hotz -- Ion-exchange chromatography / David Sheehan -- Size-exclusion chromatography / Paul Cutler -- Hydrophobic interaction chromatography / Paul A. O'Farrell -- Affinity chromatography / Adam Charlton and Michael Zachariou -- High performance liquid chromatography (HPLC) of peptides and proteins / Tzong-Hsien Lee and Marie-Isabel Aguilar -- Amino acid analysis / Ian Davidson and Paula O'Connor -- Surface plasmon resonance / K. Scott Phillips and Quan Jason Cheng -- Macromolecular crystallography / Bernhard Rupp and Katherine A. Kantardjieff --  Microchip devices for bioanalysis / Anna C. Kinsella and Shelley D. Minteer -- Mammalian cell culture / Simon P. Langdon -- Plant tissue culture / Victor M. Loyola-Vargas ... [et al.] -- Stem cells and regenerative medicine / Mohan C. Vemuri and Chellu S. Chetty -- Cryopreservatioin : conservation of bioresources at ultra low temperatures / John G. Day ... [et al.] -- Magnetic resonance imaging / Pottumarthi V. Prasad and Pippa Storey -- Electron microscopy / John Kuo -- Confocal microscopy / Guy Cox -- Laser microdissection / Graeme I. Murray -- Biomedical uses of flow cytometry / James L. Weaver and Maryalice Stetler-Stevenson -- Immunomicroscopy / Constance Oliver -- In situ hybridization / Ian A. Darby and Tim D. Hewitson -- High throughput screening / William P. Janzen</tableOfContents>
  <targetAudience authority="marctarget">specialized</targetAudience>
  <note type="statement of responsibility">edited by John M. Walker, Ralph Rapley</note>
  <note>Includes bibliographical references and index</note>
  <subject authority="lcsh">
    <topic>Molecular biology</topic>
    <topic>Methodology</topic>
    <topic>Handbooks, manuals, etc</topic>
  </subject>
  <subject authority="mesh">
    <topic>Genetic Techniques</topic>
  </subject>
  <subject authority="mesh">
    <topic>Cytological Techniques</topic>
  </subject>
  <subject authority="mesh">
    <topic>Molecular Biology</topic>
  </subject>
  <identifier type="isbn">9781603273701 (hbk.)</identifier>
  <identifier type="isbn">1603273700 (hbk.)</identifier>
  <identifier type="isbn">9781603273749 (pbk.)</identifier>
  <identifier type="isbn">1603273743 (pbk.)</identifier>
  <identifier type="uri">http://www.loc.gov/catdir/toc/fy0903/2008926601.html</identifier>
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    <url displayLabel="Table of contents only">http://www.loc.gov/catdir/toc/fy0903/2008926601.html</url>
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    <recordCreationDate encoding="marc">090618</recordCreationDate>
    <recordChangeDate encoding="iso8601">20251117141250.0</recordChangeDate>
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