03275nam a2200313 a 4500 vtls000046834 KUKTEM 20251117141306.0 100701t2009 flua f b 001 0 eng d 9780849372278 (hardcover : alk. paper) 201107132355 VLOAD 201011021559 fauzi 201011021539 fauzi 201007011643 shikin UMP Handbook of cryo-preparation methods for electron microscopy / edited by Annie Cavalier, Danièle Spehner, Bruno M. Humbel Boca Raton : CRC Press, c2009 xix, 682 p. : ill. ; 26 cm. Methods in visualization Includes bibliographical references and index Slam-freezing, metal-mirror freezing / Danièle Spehner and Ludwig Edelmann -- Plunge-freezing (Holey carbon method) / Sacha De Carlo -- Controlled vitrification / Peter M. Frederik, Felix de Haas, and Marc M.H. Storms -- BAL-TEC HPM 010 high-pressure freezing machine / Andres Kaech -- High-pressure freezing Leica EMPACT / Dimitri Vanhecke and Daniel Studer -- Frozen-hydrated macromolecules for structural analysis / Corinne Crucifix, Gabor Papai and Patrick Schultz -- Two-dimensional crystals / Patrick Bron and Janet Vonck -- Cryo-negative staining / Sacha De Carlo -- Vitrification of dynamic microtubules / Isabelle Arnal, Marine Malleter, and Denis Chrétien -- CEMOVIS : cryo-electron microscopy of vitreous sections / Jacques Dubochet ... [et al.] -- Cryo-electron tomography / Andrew Leis, Manuela Gruska, and Wolfgang Baumeister -- Freeze-substitution / Bruno M. Humbel -- Cryo-fixation, freeze-substitution, rehydration and Tokuyasu cryo-sectioning / York-Dieter Stierhof ... [et al.] -- Freeze-drying and embedding of biological material / Ludwig Edelmann -- The shadow of hydrated biological specimens / Daniel Thomas -- Cryo-fracture of self assembled systems in organic solvent / Marc Schmutz and Philippe J. Mésini -- Progressive lowering of temperature for immunolabeling and in situ hybridization / Annie Cavalier and Danièle Spehner -- Cryo-sectioning according to Tokuyasu / Bruno M. Humbel and York-Dieter Stierhof -- Cryo-preparation procedures for elemental imaging by SIMS and EFTEM / Christian Bordat and Jean-luc Guerquin-Kern -- Correlative light and electron microscopy / Heinz Schwarz and Bruno M. Humbel -- SDS-digested freeze-fracture replica labeling (SDS-FRL) / Yugo Fukazawa ... [et al.] -- Immunolabeling of ultrathin sections with enlarged 1 nm gold or QDots / York-Dieter Stierhof -- 3-D electron tomography of cells and organelles / Willie J.C. Geerts, Bruno M. Humbel and Arie J. Verkleij -- Final considerations / Hellmuth Sitte Cryobiology Methodology Electron microscopy Methodology Cryopreservation methods Laboratory Manuals Microscopy, Electron methods Laboratory Manuals Cavalier, Annie Spehner, Daniele Humbel, Bruno M. Methods in visualization series Table of contents only http://www.loc.gov/catdir/toc/fy0903/2008016244.html 0 0 0 1 10000 10000 2019-09-04 573.99 0 QH324.9.C7 H36 2009 r 0000051255 2019-09-04 00:00:00 1 573.99 2019-09-04 REF VIRTUA40 14397 14403 VTLSSORT0080*0200*0400*0900*2450*2600*3000*4900*5040*5050*6500*6501*6502*6503*7000*7001*7002*8300*8560*9991