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  <titleInfo>
    <title>Handbook of cryo-preparation methods for electron microscopy</title>
  </titleInfo>
  <name type="personal">
    <namePart>Cavalier, Annie</namePart>
  </name>
  <name type="personal">
    <namePart>Spehner, Daniele</namePart>
  </name>
  <name type="personal">
    <namePart>Humbel, Bruno M.</namePart>
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  <typeOfResource>text</typeOfResource>
  <genre authority="marc">bibliography</genre>
  <originInfo>
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    <place>
      <placeTerm type="text">Boca Raton</placeTerm>
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    <publisher>CRC Press</publisher>
    <dateIssued>c2009</dateIssued>
    <dateIssued encoding="marc">2009</dateIssued>
    <issuance>monographic</issuance>
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  <language>
    <languageTerm authority="iso639-2b" type="code">eng</languageTerm>
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  <physicalDescription>
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    <extent>xix, 682 p. : ill. ; 26 cm.</extent>
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  <tableOfContents>Slam-freezing, metal-mirror freezing / Danièle Spehner and Ludwig Edelmann -- Plunge-freezing (Holey carbon method) / Sacha De Carlo -- Controlled vitrification / Peter M. Frederik, Felix de Haas, and Marc M.H. Storms -- BAL-TEC HPM 010 high-pressure freezing machine / Andres Kaech -- High-pressure freezing Leica EMPACT / Dimitri Vanhecke and Daniel Studer -- Frozen-hydrated macromolecules for structural analysis / Corinne Crucifix, Gabor Papai and Patrick Schultz -- Two-dimensional crystals / Patrick Bron and Janet Vonck -- Cryo-negative staining / Sacha De Carlo -- Vitrification of dynamic microtubules / Isabelle Arnal, Marine Malleter, and Denis Chrétien -- CEMOVIS : cryo-electron microscopy of vitreous sections / Jacques Dubochet ... [et al.] -- Cryo-electron tomography / Andrew Leis, Manuela Gruska, and Wolfgang Baumeister -- Freeze-substitution / Bruno M. Humbel -- Cryo-fixation, freeze-substitution, rehydration and Tokuyasu cryo-sectioning / York-Dieter Stierhof ... [et al.] -- Freeze-drying and embedding of biological material / Ludwig Edelmann -- The shadow of hydrated biological specimens / Daniel Thomas -- Cryo-fracture of self assembled systems in organic solvent / Marc Schmutz and Philippe J. Mésini -- Progressive lowering of temperature for immunolabeling and in situ hybridization / Annie Cavalier and Danièle Spehner -- Cryo-sectioning according to Tokuyasu / Bruno M. Humbel and York-Dieter Stierhof -- Cryo-preparation procedures for elemental imaging by SIMS and EFTEM / Christian Bordat and Jean-luc Guerquin-Kern -- Correlative light and electron microscopy / Heinz Schwarz and Bruno M. Humbel -- SDS-digested freeze-fracture replica labeling (SDS-FRL) / Yugo Fukazawa ... [et al.] -- Immunolabeling of ultrathin sections with enlarged 1 nm gold or QDots / York-Dieter Stierhof -- 3-D electron tomography of cells and organelles / Willie J.C. Geerts, Bruno M. Humbel and Arie J. Verkleij -- Final considerations / Hellmuth Sitte</tableOfContents>
  <targetAudience authority="marctarget">specialized</targetAudience>
  <note type="statement of responsibility">edited by Annie Cavalier, Danièle Spehner, Bruno M. Humbel</note>
  <note>Includes bibliographical references and index</note>
  <subject authority="lcsh">
    <topic>Cryobiology</topic>
    <topic>Methodology</topic>
  </subject>
  <subject authority="lcsh">
    <topic>Electron microscopy</topic>
    <topic>Methodology</topic>
  </subject>
  <subject authority="mesh">
    <topic>Cryopreservation</topic>
    <topic>methods</topic>
    <topic>Laboratory Manuals</topic>
  </subject>
  <subject authority="mesh">
    <topic>Microscopy, Electron</topic>
    <topic>methods</topic>
    <topic>Laboratory Manuals</topic>
  </subject>
  <relatedItem type="series">
    <titleInfo>
      <title>Methods in visualization series</title>
    </titleInfo>
  </relatedItem>
  <identifier type="isbn">9780849372278 (hardcover : alk. paper)</identifier>
  <identifier type="uri">http://www.loc.gov/catdir/toc/fy0903/2008016244.html</identifier>
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    <recordCreationDate encoding="marc">100701</recordCreationDate>
    <recordChangeDate encoding="iso8601">20251117141306.0</recordChangeDate>
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