02332nam a2200253 a 4500 vtls000031343 KUKTEM 20251114204415.0 080902t2008 my a f m 000 0 eng|d THE0004136(Local) 201905132121 farhana 201107132241 VLOAD 200908141515 VLOAD 200908141445 VLOAD 200809021030 ida84 UMP TP156.F4 R67 2008 rs Thesis Rosmerah Liaw Optimization of fermentation for biopolymer production in shake-flask / Rosmerah Liaw Optimization of fermentation for biopolymer production in shake-flask [electronic resource] Kuantan, Pahang : UMP, 2008 36 p. : ill. (some col.) ; 30 cm. + 1 computer disc Project paper (Bachelor of Chemical Engineering) --- Universiti Malaysia pahang - 2008 Polyhydroxyalkanoates (PHA) are linear polyester produced in nature by bacterial fermentation of sugar or lipids that was first discovered by Lemoigne in 1925. Poly-Β-Hydroxybutyrate (PHB) is the most well known member of the PHAs. PHB is natural, biodegradable polyester, which is accumulated in the form of intracellular granules by a large variety of bacteria. These granules act as energy reserve materials when nutrients such as nitrogen and phosphorus source are available in limiting concentration in the presence of excess carbon source. This research studies the effect of variables in optimizing the production of PHB from bacteria Cupria vidus necator. In this study, the variables that being studied is temperature (28°C-35°C), glucose concentration (10g/L – 30g/L) and agitation speed (150rpm-300rpm). The purpose of this study is to find the value of media variable that will optimize the production of PHB in the fermentation process of biopolymer production. From the experiment and calculation, the maximum yield of biomass and PHB is achieve at the temperature of 34C, glucose concentration of 15.63g/L and at agitation of 222.83rpm. The maximum predicted yield for biomass (cell dry mass) is 3.07855g/L and the maximum predicted yield for PHB is 0.564093g/Lo. -Author Fermentation Biopolymers 0 0 0 1 10000 10000 2019-09-04 0 TP156.F4 R67 2008 rs Thesis 0000031409 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 2768 | TP156.F4 R67 2008 rs Thesis 0000031410 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 1509 1515 VTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5200*6500*6501*9992