02618nam a2200277 a 4500001001400000003000700014005001700021008004100038020002200079039011000101040000800211090003200219100001800251245009100269246009700360260003400457300005900491502009100550520129700641650001701938650001601955952012601971952013602097999002502233999008202258vtls000031343KUKTEM20251114204415.0080902t2008    my a   f m    000 0 eng|d  aTHE0004136(Local) 9a201905132121bfarhanac201107132241dVLOADc200908141515dVLOADc200908141445dVLOADy200809021030zida84  aUMP  aTP156.F4 R67 2008 rs Thesis0 aRosmerah Liaw10aOptimization of fermentation for biopolymer production in shake-flask /cRosmerah Liaw3 aOptimization of fermentation for biopolymer production in shake-flaskh[electronic resource]  aKuantan, Pahang :bUMP,c2008  a36 p. :bill. (some col.) ;c30 cm. +e1 computer disc  aProject paper (Bachelor of Chemical Engineering) --- Universiti Malaysia pahang - 20083 aPolyhydroxyalkanoates (PHA) are linear polyester produced in nature by bacterial fermentation of sugar or lipids that was first discovered by Lemoigne in 1925. Poly-Β-Hydroxybutyrate (PHB) is the most well known member of the PHAs. PHB is natural, biodegradable polyester, which is accumulated in the form of intracellular granules by a large variety of bacteria. These granules act as energy reserve materials when nutrients such as nitrogen and phosphorus source are available in limiting concentration in the presence of excess carbon source. This research studies the effect of variables in optimizing the production of PHB from bacteria Cupria vidus  necator. In this study, the variables that being studied is temperature (28°C-35°C), glucose concentration (10g/L – 30g/L) and agitation speed (150rpm-300rpm). The purpose of this study is to find the value of media variable that will optimize the production of PHB in the fermentation process of biopolymer production. From the experiment and calculation, the maximum yield of biomass and PHB is achieve at the temperature of 34C, glucose concentration of 15.63g/L and at agitation of 222.83rpm. The maximum predicted yield for biomass (cell dry mass) is 3.07855g/L and the maximum predicted yield for PHB is 0.564093g/Lo. -Author 0aFermentation 0aBiopolymers  00104071a10000b10000d2019-09-04l0oTP156.F4 R67 2008 rs Thesisp0000031409r2019-09-04 00:00:00t1w2019-09-04yPSM  00104071a10000b10000d2019-09-04l0oCD 2768 | TP156.F4 R67 2008 rs Thesisp0000031410r2019-09-04 00:00:00t1w2019-09-04yPSM  aVIRTUA40c1509d1515  aVTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5200*6500*6501*9992