01786nam a2200241 a 4500 vtls000057155 KUKTEM 20251114204417.0 111212t2010 my dao f m 000 0 eng d THE0004145(Local) 201905132129 farhana 201112121539 Fida UMP TP156.F5 Z53 2010 rs Bc. Zianor Azrina Zianon Abdin Whey protein fractionation based on q-sepharose anion exchange chromatography / Zianor Azrina Zianon Abdin Kuantan, Pahang : UMP, 2010 xi, 50 p. : ill. (some col.) ; 30 cm. + 1 computer disc Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2010 Bibliography: p. [40]-41 Whey proteinsarewell known for their nutritional value and had versatile functional properties.The mains proteins present in whey are α-lactalbumin, βlactoglobulin, immunoglobulin andbovin eserum albumin. In this study, whey protein component is separate into several fractions using Q-Sepharose anion exchange chromatography column. The chromatographic process was run at different pH range from pH5 to pH8. The best separation was achieved at pH6 in which the major protein component can be separated into their individual protein fraction especially contains αlactalbumin and β-lactoglobulin. The yield of α-lactalbumin and β-lactoglobulin recovered from 2 ml whey at pH 6 was 46% and 98.4% respectively. Fractionation of single and pure protein component from whey can add the value of the wheybased product and these proteins can be used for specific application. Ultrafiltration 0 0 0 1 10000 10000 2019-09-04 0 TP156.F5 Z53 2010 rs Bc. 0000060228 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 5505 | TP156.F5 Z53 2010 rs Bc. 0000060229 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 1569 1575 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992