02446nam a2200241 a 4500 vtls000045140 KUKTEM 20251114204422.0 100219t2009 my ao f m 000 0 eng d THE0002290(Local) 201905241223 shamsul 201108030917 Fida 201107132307 VLOAD 201002191023 kam UMP QR186.85 .S53 2009 rs Bc. Mohd Shamsul Husin Simulation study of monoclonal antibody production using superpro, upstream process / Mohd Shamsul Bin Husin Simulation study of monoclonal antibody production using superpro, upstream process [computer file] Kuantan : UMP, 2009 30 p. : ill. (some col.) ; 30 cm. + 1 computer disc Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2009 The purpose of this study is to approximate hybridoma growth kinetic model by comparing simulation result from SuperPro Designer® and experimental result. Modeling of hybridoma include calculation of mass for 1 cell and its density. Two kinetic models tested in this study; experimental correlation and de Tremblay et al. (1992). Simplification need to be made as this will allow selected model to be used in SPD. Value of μmax, KsGLN, KsGLC are 1.09 d-1 (0.05 h-1), 0.3 mM (43.85 mg/L) and 0.1 mM (18.02 mg/L) respectively for de Tremblay and for experimental correlation, their values are 0.158 h-1, 0.0016 mM (0.23 mg/L) and 12.05 mM (2170.93 mg/L) respectively. Experimental data shows no stationary phase but simulation results show stationary phase. From simulation, cell count of 195907.7 while final concentration of glucose, glutamine, ammonia and lactate are 16.38, 4.07, 3.01 and 5.59 mmol/L respectively (experimental correlation) and cell count of 196185.73 while final concentration of glucose, glutamine, ammonia and lactate are 10.73, 2.09, 6.07 and 11.30 mmol/L respectively (de Tremblay). Serious deviations occurred because of simplification on de Tremblay model and inaccurate prediction of glutamine effect on hybridoma’s growth. Metabolic reaction used without taking hybridoma real behavior into account. Simulation shows exponential phase without having going through lag phase. Monoclonal antibodies 0 0 0 1 10000 10000 2019-09-04 0 QR186.85 .S53 2009 rs Bc. 0000042961 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 4012 | QR186.85 .S53 2009 rs Bc. 0000042962 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 1708 1714 VTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5200*6500*9992