02189nam a2200193 a 4500001001400000003000700014005001700021008004100038020002200079040000800101100002700109245016100136246015100297260002600448300004700474502010600521520135600627650001201983vtls000045103KUKTEM20251114204422.0100205t2009    my a   f      000 0 eng d  aTHE0004762(Local)  aUMP0 aSiti Nor Shadila Alias10aEffects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis :ba statistical approach /cSiti Nor Shadila Binti Alias3 aEffects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysish[electronic resource] : a statistical approach  aKuantan :bUMP,c2009  a41 p. :bill. ;c30 cm. +e1 computer disc  aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 20093 aThe aim of this study was to maximize the glucose concentration produced from enzymatic hydrolysis of tapioca starch. The tapioca starch was enzymatically hydrolyzed using α-amylase from Bacillus lichenformis followed by amyloglucosidase action from Aspergillus niger. Effects of liquefaction temperature (X1), saccharification temperature (X2), liquefaction pH (X3) and saccharification pH (X4¬) were evaluated. 24 full factorial design with 1 replicate and 3 centered point was applied to determine the significant parameters affecting the production of glucose concentration. The range of the factors employed were 60 -90˚C (liquefaction temperature), 40-60˚C (saccharification temperature), 5-7 (liquefaction pH) and 4-6 (saccharification pH). The maximum glucose concentration obtained experimentally was 329.10 g/L. The ANOVA shows that the effects of liquefaction temperature and saccharification pH on glucose production were very significant. The saccharification temperature and liquefaction pH, on the other hand did not influence the glucose production. The optimum liquefaction temperature, saccharification temperature, liquefaction pH and saccharification pH suggested by the design of experiment were 90˚C, 60˚C, 7 and 6 respectively. From that optimum condition, the maximum glucose concentration of 331.8 g/L was estimated.     0aGlucose