02448nam a2200241 a 4500 vtls000045103 KUKTEM 20251114204422.0 100205t2009 my a f 000 0 eng d THE0004762(Local) 201905151058 smfh 201107132306 VLOAD 201002081443 kam 201002051711 kam UMP TP414 .S66 2009 rs Bc. Siti Nor Shadila Alias Effects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis : a statistical approach / Siti Nor Shadila Binti Alias Effects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis [electronic resource] : a statistical approach Kuantan : UMP, 2009 41 p. : ill. ; 30 cm. + 1 computer disc Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009 The aim of this study was to maximize the glucose concentration produced from enzymatic hydrolysis of tapioca starch. The tapioca starch was enzymatically hydrolyzed using α-amylase from Bacillus lichenformis followed by amyloglucosidase action from Aspergillus niger. Effects of liquefaction temperature (X1), saccharification temperature (X2), liquefaction pH (X3) and saccharification pH (X4¬) were evaluated. 24 full factorial design with 1 replicate and 3 centered point was applied to determine the significant parameters affecting the production of glucose concentration. The range of the factors employed were 60 -90˚C (liquefaction temperature), 40-60˚C (saccharification temperature), 5-7 (liquefaction pH) and 4-6 (saccharification pH). The maximum glucose concentration obtained experimentally was 329.10 g/L. The ANOVA shows that the effects of liquefaction temperature and saccharification pH on glucose production were very significant. The saccharification temperature and liquefaction pH, on the other hand did not influence the glucose production. The optimum liquefaction temperature, saccharification temperature, liquefaction pH and saccharification pH suggested by the design of experiment were 90˚C, 60˚C, 7 and 6 respectively. From that optimum condition, the maximum glucose concentration of 331.8 g/L was estimated. Glucose 0 0 0 1 10000 10000 2019-09-04 0 CD 3925 | TP414 .S66 2009 rs Bc. 0000042796 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 TP414 .S66 2009 rs Bc. 0000042795 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 1711 1717 VTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5200*6500*9992