02225nam a2200253 a 4500 vtls000045108 KUKTEM 20251114204423.0 100208t2009 my a f 000 0 eng d THE0004758(Local) 201905151054 smfh 201203131102 ida 201108030932 Fida 201107132306 VLOAD 201002081434 ida UMP TP414 .N34 2009 rs Bc. Ahmad Nahri Abdul Aziz Effect of enzyme loading and time on production of sugar from tapioca starch using enzymatic hydrolysis : a statistical approach/ Ahmad Nahri Abdul Aziz Effect of enzyme loading and time on production of sugar from tapioca starch using enzymatic hydrolysis [computer file]: a statistical approach Kuantan, Pahang : UMP, 2009 42 p. : ill. (some col.) ; 30 cm. + 1 computer disc Project paper (Bachelor of Chemical Engineering (Biotechnoogy)) -- Universiti Malaysia Pahang - 2009 Bibliography : p.[34]-36 Two-step enzymatic hydrolysis of tapioca starch by commercially available α-amylase and glucoamylase was carried out in this study. Effects of enzyme loading and hydrolysis time for liquefaction and saccharification process of enzymatic hydrolysis on production of sugar from tapioca starch were investigated. A statistical approach was employed to study the effect of selected parameters where two level factorial design was applied for the experimental design. From the result, it is shown that the highest glucose concentration achieved was 277.80g/L. From the analysis of variance (ANOVA), it is found that the liquefaction time and the interaction effect between α-amylase loading and saccharification time show significant effects on the enzymatic hydrolysis. The optimum conditions suggested by the design of experiment were: 80μL α-amylase loading, 5.75mg glucoamylase loading 5.75mg, liquefaction time 1 hour and saccharification time 4 hours. From this condition, an amount of high glucose concentration was estimated.-Author- Glucose 0 0 0 1 10000 10000 2019-09-04 0 TP414 .N34 2009 rs Bc. 0000042997 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 4030 | TP414 .N34 2009 rs Bc. 0000042998 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 1720 1726 VTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5040*5200*6500*9992