02389nam a2200229 a 4500001001400000003000700014005001700021008004100038020002200079040000800101100003000109245015600139246015000295260003400445300006300479502009000542504003000632520142600662650002402088650002702112650002002139vtls000045156KUKTEM20251114204423.0100222t2009    my a   f m    001 0 eng|d  aTHE0004428(Local)  aUMP0 aMohd Khairul Afizan Harun10aSeparation of fructosyltransferase using ultrafiltration membrane :beffect of pH and ionic strength on flux and rejection /cMohd Khairul Afizan Harun3 aSeparation of fructosyltransferase using ultrafiltration membrane :beffect of pH and ionic strength on flux and rejectionh[electronic resource]  aKuantan, Pahang :bUMP,c2009  aix, 98 p. :bill. (some col.) ;c30 cm. +e1 computer disc  aProject paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2009  aBibliography : p. [58]-603 aThere  are  various  methods  to  separate  between  macro  molecule  and  non dissolved particle in chemical process. One of the methods is separation process using ultrafiltarion membrane.  In  filtration  process,  the macromolecules  such  as  enzyme will  be  retained  on  the membrane  surface.  This  experiment  is  study  about  fouling characteristic occur  in  an  industry. The  fouled membrane  surface problem gives  the high  cost  operation  and  reduces  the  quality  of  production. Therefore,  the  main objectives for this experiment are to determine the effect of pH and ionic strength on membrane  flux  and  rejection during  fructosyltransferase  (FTase)  separation. The 50 kDa molecular weight cut off (MWCO) of ultrafiltration membrane was used during this experiment. Cross flow filtration was used to run this experiment in the lab scale. Total  organic  carbon  (TOC)  was  used  to  analysis  the  concentration  of  sample.   Potassium  dihydrogen  phosphate (KH2PO4)  and  dipotassium  hydrogen  phosphate buffer solution range pH 5 to pH 8 was applied to find the effect of pH and various  molarities  of  NaCl  (0.5M  to  2.0M)  was  used  to  find  the  effect  of  ionic strength in ultrafiltration membrane. The experimental result shows that the optimum pH and ionic strength was 8.0 and 0.5M, respectively,  in order to separate the FTase solution using ultrafiltration membrane. 0aMembrane separation 0aMembranes (Technology) 0aUltrafiltration