02664nam a2200253 a 4500 vtls000045473 KUKTEM 20251114204424.0 100430t2010 my a f m 000 0 eng dd THE0002288(Local) 201905241221 shamsul 201108030915 Fida 201107132316 VLOAD 201004301112 ida 201004301056 ida UMP QR186.85 .I43 2009 rs Bc. Muhamad Nur Iman Ahmad Razali A simulation study on downstream processing of monoclonal antibodies production using superpro designer / Muhamad Nur Iman Bin Ahmad Razali A simulation study on downstream processing of monoclonal antibodies production using superpro designer [computer file] Kuantan, Pahang : UMP, 2009 xiv, 53 p. : ill. (some col.) ; 30 cm. + 1 computer disc Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009 Bibliography : p. 51-52 The production of monoclonal antibodies and Fc fusion proteins form the largest and most rapidly expanding category of the biopharmaceuticals today with the annual sales exceeding USD$8 billion with the application across a wide range of diseases especially for the immunotherapy purpose. With the growth of this class of biomolecules, significant thought on the most efficient downstream processing steps for monoclonal antibodies has been taken into the highly consideration by the manufacturer in order to obtain the highest yield with the highest purity of the product which is why the simulation study are crucial in this development. The objectives of this study are to propose the most suitable framework of downstream processing for monoclonal antibodies production in order to achieve highest purities of mAbs and to stimulate the propose framework using SuperPro Designer® simulator. The process was initially done via the disk stack centrifuge and followed by microfiltration in order to harvest the protein. Three column of chromatography (Protein A affinity, anion exchange and hydrophobic interaction) were used to obtain the highest purity of the therapeutic. Viral inactivation also was employed to free the product from the endotoxins and pyrogens. The propose steps then, was introduced into the SuperPro Designer® simulator and the result obtained were 100% purity of IgG with 9.49345 g/L of concentration and 0.16687 kg/batch with 76.3% of recovery of the product. Monoclonal antibodies 0 0 0 1 10000 10000 2019-09-04 0 QR186.85 .I43 2009 rs Bc. 0000042957 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 4010 | QR186.85 .I43 2009 rs Bc. 0000042958 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 1759 1765 VTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5040*5200*6500*9992