02542nam a2200253 a 4500 vtls000045481 KUKTEM 20251114204424.0 100430t2009 my a f m 000 0 eng d THE0002186(Local) 201905131712 yusri 201112051409 Fida 201108030912 Fida 201107132316 VLOAD 201004301516 ida UMP QD702.C5 N67 2009 rs Bc. Norheziwan Hamsah Optimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology / Norheziwan Bin Hamsah Kuantan, Pahang : UMP, 2009 xiv, 56 p. : ill. (some col.) ; 30 cm. + 1 computer disc Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009 Bibliography : p. [42]-49 The function of chitinase as a chitin digestive enzyme has attracted the attention as one of the potential materials for control of phytopathogenic fungi and insect pests. However, chitinase is uneconomic for commercialized due to the high cost in its production. The development in recombinant DNA technology has helped to reduce chitinase production cost and enhance its production. The aim of this study is to determine the optimum conditions toward the production of recombinant chitinase in Escherichia coli by manipulating the concentration of inducers (IPTG and lactose). The best concentration of IPTG and lactose as inducers were determined by using the conventional method. Results from conventional method were used for further improvement of optimization by using Response Surface Methodology (RSM). In Response Surface Methodology, the optimization of expression was carried out based on the Central Composite Design (CCD). The effect of the inducers concentration toward the expression level of the recombinant chitinase production were analyzed and optimum values of tested variables for the production of chitinase were 1.19 mM IPTG and 0.98% lactose. Response surface analysis revealed that 0.513 U/ml of chitinase activity was achieved by using the optimized condition. This improved about 13.2-fold compare to initial experiment which produced 0.039 U/ml. This showed that combination between IPTG and lactose has increased the production of recombinant chitinase. Chitin Response surfaces (Statistics) 0 0 0 1 10000 10000 2019-09-04 0 QD702.C5 N67 2009 rs Bc. 0000042939 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 4001 | QD702.C5 N67 2009 rs Bc. 0000042940 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 1765 1771 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992