02831nam a2200277 a 4500001001400000003000700014005001700021008004100038020002200079039004500101040000800146090002900154100001600183245010400199260003400303300004500337502010600382504002800488520164000516650001602156650001802172952012302190952013302313999002502446999008202471vtls000057504KUKTEM20251114204435.0111216t2010    my a   f m    001 0 eng d  aTHE0004129(Local) 9a201905132116bfarhanay201112161550zida  aUMP  aTP156.F4 M39 2010 rs Bc.0 aMazia Awang10aOptimization of biopolymer production in shake flask using oil palm trunk sap medium /cMazia Awang  aKuantan, Pahang :bUMP,c2010  axiv, 81 p. :bill. ;c30 cm. +e1 CD-ROM  aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010  aBibliography : p. 44-453 aPolyhydroxybutyrate (PHB) is a biodegrable polymer which is an intracellular product produce by a bacteria. This research is conducted to optimize the biopolymer production in shake flask using oil palm trunk sap medium with Cupriavidus necator. It consists of four major phases starting with the innoculum development process, followed by the fermentation process, the analysis of the process and the process optimization. The innoculum development  process is done to produce the 50 ml of innoculum Cupriavidus necator  in shake flask by using growth media and incubated in incubator shaker with set point values for agitation speed and temperature. This experiment is followed with the fermentation process involving the manipulation of the parameters agitation speed, temperature and percent volume of  oil palm trunk sap. Among the experimental points, it is found that the highest yield of PHB is produced in conditions of 150 RPM agitation speed, 32oC temperature and 20% volume of oil palm trunk sap. This experiment was followed by mathematical analysis using Yates’ Method to study the main effects and interactive effects of changing the level of experimental variables. The calculation showed that percent volume of oil palm trunk sap has a significant main effect on PHB production while the significant interactive effect is the combination of agitation speed and percent volume of oil palm trunk sap . From the experiment for Method of the Path of Steepest Ascent the highest production of PHB occured at parameter 31.1 oC for temperature, 138 RPM for agitation speed and 33.8% for percent volume of oil palm trunk sap 0aBiopolymers 0aFermentations  00104071a10000b10000d2019-09-04l0oTP156.F4 M39 2010 rs Bc.p0000061024r2019-09-04 00:00:00t1w2019-09-04yPSM  00104071a10000b10000d2019-09-04l0oCD 5675 | TP156.F4 M39 2010 rs Bc.p0000061025r2019-09-04 00:00:00t1w2019-09-04yPSM  aVIRTUA40c2070d2076  aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992