01956nam a2200253 a 4500 vtls000058553 KUKTEM 20251114204449.0 120305t2010 my da f m 000 0 eng d THE0004133(Local) 201905132119 farhana 201203051313 ida 201203051106 Fida UMP TP156.F4 N67 2010 rs Bc. Nik Nor Aziati Abd Aziz Effect of substrate concentration and ph on succinic acid production / Nik Nor Aziati Abd Aziz Kuantan, Pahang : UMP, 2010 xv, 95 p. : ill. (some col.) ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010 Bibliography: p. 60-62 The purpose of this research is to study the effect of substrate concentration and pH on succinic acid production. Succinic acid has been produced from the fermentation process by using Escherichia coli b-strain. The experiments were conducted in Modified Schott Bottle in incubator shaker to maintain anaerobic condition, temperature and agitation speed. Fermentation were carried out at the optimum temperature of E.coli growth which is 37 OC for 72 hours. The results of HPLC analysis showed that increase in fermentation time will increase succinic acid production. The maximum production of succinic acid is at pH 6.0. Initial substrate concentration (5 to 15 g/L) will increase Succinic acid production, but after 15 g/L substrate concentration, the succinic acid production will decrease. From this study, it was observed that the substrate concentration and pH are important factor that affects both growth and growth-associated productions of molecules to increased succinic acid production. Fermentation Succinic acid 0 0 0 1 10000 10000 2019-09-04 0 TP156.F4 N67 2010 rs Bc. 0000063206 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 5876 | TP156.F4 N67 2010 rs Bc. 0000063207 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 2486 2492 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992