02841nam a2200265 a 4500 vtls000045113 KUKTEM 20251114204455.0 100209t2009 my a f m 000 0 eng|d THE0004525(Local) 201905131532 azhar 201112291324 ida 201108030933 Fida 201107132307 VLOAD 201002091355 ida UMP TP248.65.P76 F37 2009 rs Bc. Farhani Faisal Protein purification using zeolite adsorbent / Farhani Faisal Protein purification using zeolite adsorbent [electronic resource] Kuantan, Pahang : UMP, 2009 80 p. : ill. (some col.) ; 30 cm. + 1 computer disc Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2009 Bibliography : p. 69-70 There has been much interest generated in the development of protein purification since many of modern biotechnology products can be produce from purified protein such as insulin, vaccines and food additives. The aim of this research is to study the most optimum condition to purify protein in order to produce a high purity protein with low cost by applying the principle adsorption process using zeolite adsorbent. Adsorption of Bovine Serum Albumin used as protein sample on Y, Beta and ZSM-5 zeolites at various pH values was carried out in sequence to investigate the effect of different pH value and different types of adsorbent on protein purification. Batch adsorption experiment was carried out by contacting the same amount of different zeolites synthesized with the Bovine Serum Albumin solution. Outcome of the experiment shows that maximum adsorption capacity occurred when Y zeolite was used as the adsorbent at pH 5 that is close to isoelectric point (pI) of Bovine Serum Albumin which is 4.7. This proves that adsorption capacity depends on the physicochemical properties of the zeolite as well as the pH of protein solution. The maximum adsorption on the zeolites tended to occur when the pH was at or just below the pI of the protein. At this pH, the protein sample is positively charged. Adsorption isotherms obtained on effect of different type of adsorbent at pH5 and effect of different pH on Y zeolite were found to be confirmed well to the ideal Langmuir model equation. In order to improve in the next study, other parameters such as protein size, temperature of the protein solution and protein concentration need to be considered as well. Protein Purification Zeolites 0 0 0 1 10000 10000 2019-09-04 0 TP248.65.P76 F37 2009 rs Bc. 0000043003 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 4033 | TP248.65.P76 F37 2009 rs Bc. 0000043004 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 2654 2660 VTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5040*5200*6500*6501*9992