02197nam a2200253 a 4500 vtls000056480 KUKTEM 20251114204507.0 111201t2010 my a f m 000 0 eng d THE0004351(Local) 201905161041 rohana 201112011121 Fida UMP TP248.E5 I99 2010 rs Bc. Izzah Suhaila Abu Bakar Extraction of papain enzyme from papaya leaves using hot water extraction with ultrasonic – assisted pretreatment / Izzah Suhaila Abu Bakar Kuantan, Pahang : UMP, 2010 xiii, 68 p. : ill. (some col.) ; 30 cm. + 1 computer disc Project paper (Bachelor of Chemical Engineering (Biotechnology)) --- Universiti Malaysia Pahang - 2010 Bibliography : p. [51]-56 Papaya leaf is not just a byproduct of papaya tree but it contains an essential enzyme which is papain. The aim of the study is to measure the amount of this enzyme extracted from leaf tissue using hot water extraction with ultrasonic-assisted pretreatment as well as to determine the optimum condition for the extraction process. The leaves were subjected to a pretreatment which is grinding and ultrasonication followed by hot water extraction and finally, enzymatic analysis using UV-Vis spectrophotometer. The parameters involved in the process were ultrasonication time (30 – 90 minutes) and frequency (18 – 26 kHz), solid-solvent ratio (1:5 – 1:25), and extraction time (1 – 5 hour) and temperature (50 - 70°C). Among these parameters, maximum concentration of papain enzyme was achieved at 60 minutes and 20 kHz of ultrasonication, solid-solvent ratio at 1:5, extraction time at 4 hours and extraction temperature at 60°C. In conclusion, the maximum concentration of papain enzyme achieved at this optimum parameter was 4.020 µM. Utilization of PEG–phosphate system for separation and purification of papain is recommended in order to get the pure papain enzyme. Enzymes Biotechnology Extraction (Chemistry) 0 0 0 1 10000 10000 2019-09-04 0 TP248.E5 I99 2010 rs Bc. 0000060274 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 5525 | TP248.E5 I99 2010 rs Bc. 0000060275 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 2982 2988 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992