02123nam a2200265 a 4500 vtls000058562 KUKTEM 20251114204507.0 120305t2010 my a f m 000 0 eng d THE0004431(Local) 201905161525 rohana 201203051440 Fida 201203051438 Fida UMP TP248.25.M46 N33 2010 rs Bc. Nadira Ab Aziz Effect of pH and ionic strength on membrane flux in the separation of escherichia coli by using cross-flow microfiltration / Nadira Ab Aziz Kuantan, Pahang : UMP, 2010 xiii, 79 p. : ill. (some col.) ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010 Bibliography : p. [70]-74 Recently, cross-flow microfiltration has been used to separate cells in continuous fermentation processes. The main objective of this study is to investigate the effect of pH and ionic strength on membrane flux during the separation of Escherichia coli. In order to achieve the objective of the study, hollow fibre membrane with molecular pore size of 0.2µm and surface area of 110 cm2 was used as the filter media. The experiment was carried out at a constant transmembrane pressure of 0.8 bar by varying five different pH values, ranging from pH 4.5 to 8.5 and five different concentration of salt, ranging from 0.1M to 0.5M. From this study, it is found that pH 6.5 with low concentration of salt was the best condition for the separation of E.coli. Increasing in pH increased the permeate flux, while high concentration of salt decreased the permeate flux. As a conclusion, the membrane used in this experiment can be used in the fermentation of E.coli for cell recycling because pH 6.5 is the optimum condition for fermentation of E.coli in its fermentation broth. Membrane separation Membranes (Technology) Ultrafiltration 0 0 0 1 10000 10000 2019-09-04 0 TP248.25.M46 N33 2010 rs Bc. 0000063212 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 5879 | TP248.25.M46 N33 2010 rs Bc. 0000063213 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 2986 2992 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*6502*9992