01949nam a2200253 a 4500 vtls000058544 KUKTEM 20251114204512.0 120302t2010 my a f m 000 0 eng d THE0004348(Local) 201905161036 rohana 201203021211 Fida 201203021210 Fida UMP TP248.E5 D53 2010 rs Bc. Nur Dianaty Nordin The extraction of papain from papaya leaves / Nur Dianaty Nordin Kuantan, Pahang : UMP, 2010 xv, 45 p. : ill. (some col.) ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering (Biotechnology)) --- Universiti Malaysia Pahang - 2010 Bibliography : p. 38-41 The use of papaya leaves to extract papain enzyme was evaluated for different extraction technique mainly using grinding method, enzyme-assisted extraction and ultrasonication. Enzyme concentration was then determined by measuring the amount of amino acid released in the reaction of protease assay. The combination of grinding method, enzyme-assisted extraction was proven to give highest yield of papain by 36.75% when compared to grinding alone. Then further purification of the extracted papain was done by using ammonium sulfate precipitation. The optimized concentration of ammonium sulfate saturation was achieved at 55% concentration in order to fully precipitate the protein. The combination of grinding, enzyme-assisted extraction and ultrasonication can be considered as a potential extraction technique to extract papain as it is more delicate towards protein extraction. Furthermore, the investigation on other source of papain such as from the petioles, stalks and stem could also be done. Enzymes Biotechnology Extraction (Chemistry) 0 0 0 1 10000 10000 2019-09-04 0 TP248.E5 D53 2010 rs Bc. 0000063285 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 6 10000 10000 2019-09-04 0 CD 5957 | TP248.E5 D53 2010 rs Bc. 0000063286 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 3119 3125 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992