02599nam a2200241 a 4500 vtls000018739 KUKTEM 20251114204512.0 070315t2006 my f m 000 0 eng d THE0004495(Local) 201905170904 rohana 201204161535 ida 201204161534 ida 201204161534 ida 200703151507 kam UMP TP248.65.C44 N39 2006 rs Thesis Mohd. Nazip Kassebullah Production of cellulase enzyme from Aspergillus terreues SUK-1 using sugar cane waste : the effect of substrate concentration and assay temperature / Mohd Nazip Bin Kassebullah Kuantan, Pahang : KUKTEM, 2006 54 p. : ill. ; 30 cm. + 1 compter disc Project paper (Bachelor of Chemical Engineering) -- Kolej Universiti Kejuruteraan & Teknologi Malaysia - 2006 Natural by product of agricultural waste can be turned to products of commercial interests such as ethanol, glucose and single cell protein. Much effort from scientists and researches all around the world has been put to extend the full use of agricultural waste. Reports of cellulase enzyme production from the bioconversion of lignocellulosics materials has much been made. However there is still much space to find the most suitable condition. In this research, sugar cane bagasse which is used as substrate is subjected to physical treatment using milling and grinding. After that, sugar cane bagasse is use as substrate in the production of cellulase enzyme using Aspergillus terreus SUK-1 fungus. Cellulase enzyme production was studied using submerged fermentation under shaking. The production was run in a lab scale using Erlenmeyer flask only. After that the enzyme was assayed at different temperature. The purpose of this research was to investigate the effect of substrate concentration on yield of cellulase and the optimum temperature where the yield of enzyme was the highest. Analysis is based on the activity of carboxymethylcellulase and xylanase enzymes. Result shows that the peak of xylanase activities was 0.011322 U/ml on day 5 using 5g SCB as substrate and fermentation temperature of 50°C while carboxymethylcellulase peak was 0.007985049 U/ml of activity using 3g SCB as substrate and fermentation temperature of 40°C. Xylanase activity was high when assayed at 50°C while carboxymethylcellulase activity was high when assayed at 40°C. Cellulase Aspergillus 0 0 0 1 10000 10000 2019-09-04 0 TP248.65.C44 N39 2006 rs Thesis 0000021672 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 2102 | TP248.65.C44 N39 2006 rs Thesis 0000021673 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 3125 3131 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5200*6500*6501*9992