01980nam a2200253 a 4500 vtls000058731 KUKTEM 20251114204516.0 120319t2010 my a f m 000 0 eng d THE0004749(Local) 201905141119 smfh 201203191333 Fida UMP TP372.55.E59 A94 2010 rs Bc. Azhari Anuar Biotransformation of phenol using immobilized polyphenol oxidase from banana stem / Azhari Anuar Kuantan, Pahang : UMP, 2010 xii, 41 p. : ill. (some col.) ; 30 cm. + 1 CD-ROM Project paper (Bachelor of of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010 Bibliography : p. 37-39 Polyphenol oxidase(PPO) is an enzyme that can easily find in most of the plants. It is a browning agent that cause from catalyzing the oxidation of various phenolic compounds to the corresponding quinones which lead to the formation of melanin pigment. PPO commonly used as biocatalyst in a biotransformation reaction and in this study is converting phenol to less harmful compound. The objective of this study was to study the basic kinetics of batch and continuous reactors. By conducting biotransformation using polyphenol oxidase extracted from banana stem. The enzyme immobilized using Ca-Alginate in the forms of beads. Five different phenol concentrations were transformed using different enzyme loadings for batch mode while different flow rates were used in continuous mode. Samples were analyzed using High Performance Liquid Chromatography (HPLC) at 254nm, using methanol and water (20:80) as mobile phase at 1mL/min. The space time of a batch reactor was determined and weight of catalyst loading was estimated for continuous reactor. Polyphenol oxidase Oxidases 0 0 0 1 10000 10000 2019-09-04 0 TP372.55.E59 A94 2010 rs Bc. 0000063271 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 5950 | TP372.55.E59 A94 2010 rs Bc. 0000063272 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 3263 3269 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992